Vesicular and non-vesicular transport feed distinct glycosylation pathways in the Golgi

G D'Angelo, T Uemura, CC Chuang, E Polishchuk, M Santoro, Henna Ohvo-Rekilä, T Sato, Di Tullio G, A Varriale, S D'Auria, T Daniele, F Capuani, L Johannes, Peter Mattjus, M Monti, P Pucci, RL Williams, JE Burke, FM Platt, A HaradaDe Matteis MA

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    105 Sitaatiot (Scopus)

    Abstrakti

    Newly synthesized proteins and lipids are transported across the Golgi complex via different mechanisms whose respective roles are not completely clear. We previously identified a non-vesicular intra-Golgi transport pathway for glucosylceramide (GlcCer)-the common precursor of the different series of glycosphingolipids-that is operated by the cytosolic GlcCer-transfer protein FAPP2 (also known as PLEKHA8) (ref. 1). However, the molecular determinants of the FAPP2-mediated transfer of GlcCer from the cis-Golgi to the trans-Golgi network, as well as the physiological relevance of maintaining two parallel transport pathways of GlcCer-vesicular and non-vesicular-through the Golgi, remain poorly defined. Here, using mouse and cell models, we clarify the molecular mechanisms underlying the intra-Golgi vectorial transfer of GlcCer by FAPP2 and show that GlcCer is channelled by vesicular and non-vesicular transport to two topologically distinct glycosylation tracks in the Golgi cisternae and the trans-Golgi network, respectively. Our results indicate that the transport modality across the Golgi complex is a key determinant for the glycosylation pattern of a cargo and establish a new paradigm for the branching of the glycosphingolipid synthetic pathway.
    AlkuperäiskieliEi tiedossa
    Sivut116–120
    Sivumäärä6
    JulkaisuNature
    Vuosikerta501
    Numero7465
    DOI - pysyväislinkit
    TilaJulkaistu - 2013
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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