TY - JOUR
T1 - System Approach for Building of Calcium-Binding Sites in Proteins
AU - Denesyuk, Alexander I.
AU - Permyakov, Sergei E.
AU - Johnson, Mark S.
AU - Denessiouk, Konstantin
AU - Permyakov, Eugene A.
PY - 2020
Y1 - 2020
N2 - We introduce five new local metal cation (first of all, Ca2+) recognition units in proteins: Clampn,(n-2), Clampn,(n-1), Clampn,n, Clampn,(n+1) and Clampn,(n+2). In these units, the backbone oxygen atom of a residue in position "n" of an amino acid sequence and side-chain oxygen atom of a residue in position "n + i" (i = -2 to +2) directly interact with a metal cation. An analysis of the known "Ca2+-bound niches" in proteins has shown that a system approach based on the simultaneous use of the Clamp units and earlier proposed One-Residue (OR)/Three-Residue (TR) units significantly improves the results of constructing metal cation-binding sites in proteins.
AB - We introduce five new local metal cation (first of all, Ca2+) recognition units in proteins: Clampn,(n-2), Clampn,(n-1), Clampn,n, Clampn,(n+1) and Clampn,(n+2). In these units, the backbone oxygen atom of a residue in position "n" of an amino acid sequence and side-chain oxygen atom of a residue in position "n + i" (i = -2 to +2) directly interact with a metal cation. An analysis of the known "Ca2+-bound niches" in proteins has shown that a system approach based on the simultaneous use of the Clamp units and earlier proposed One-Residue (OR)/Three-Residue (TR) units significantly improves the results of constructing metal cation-binding sites in proteins.
KW - system approach
KW - building kit
KW - calcium
KW - cation
KW - InFlames
U2 - 10.3390/biom10040588
DO - 10.3390/biom10040588
M3 - Article
SN - 2218-273X
VL - 10
JO - Biomolecules
JF - Biomolecules
IS - 4
M1 - 588
ER -