Studies Related to Norway Spruce Galactoglucomannans: Chemical Synthesis, Conformation Analysis, NMR Spectroscopic Characterization, and Molecular Recognition of Model Compounds

Filip S. Ekholm, Ana Ardá, Patrik Christoffer Eklund, Sabine André, Hans-Joachim Gabius, Jesús Jiménez-Barbero, Reko Leino

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    23 Sitaatiot (Scopus)

    Abstrakti

    Galactoglucomannan (GGM) is a polysaccharide mainly consisting of mannose, glucose, and galactose. GGM is the most abundant hemicellulose in the Norway spruce (Picea abies), but is also found in the cell wall of flax seeds, tobacco plants, and kiwifruit. Although several applications for GGM polysaccharides have been developed in pulp and paper manufacturing and the food and medical industries, attempts to synthesize and study distinct fragments of this polysaccharide have not been reported previously. Herein, the synthesis of one of the core trisaccharide units of GGM together with a less-abundant tetrasaccharide fragment is described. In addition, detailed NMR spectroscopic characterization of the model compounds, comparison of the spectral data with natural GGM, investigation of the acetyl-group migration phenomena that takes place in the polysaccharide by using small model compounds, and a binding study between the tetrasaccharide model fragment and a galactose-binding protein (the toxin viscumin) are reported.
    AlkuperäiskieliEi tiedossa
    Sivut14392–14405
    Sivumäärä14
    JulkaisuChemistry - A European Journal
    Vuosikerta18
    Numero45
    DOI - pysyväislinkit
    TilaJulkaistu - 2012
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

    Keywords

    • conformation analysis
    • galactoglucomannan
    • molecular recognition
    • NMR spectroscopy
    • oligosaccharide synthesis

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