Present study deals with two spectrophotometric methods, Conventional Spectrophotometric Estimation (Method I) and Area Under Curve Method (Method II) for quantitation of Cefprozil by using five different hydrotropic agents. These include Potassium acetate (6.0M), Potassium citrate (1.5M), Sodium acetate (4.0M), Sodium citrate (1.25M) and Urea (10.0M). All these agents do not show absorbance above 245 nm and hence do not interfere with absorbance of Cefprozil (λmax- 280 nm). Area under curve method was based on measurement of area under curve in the wavelength range 255 nm to 305 nm. Linearity of Cefprozil was found in the concentration range 10 to 60μg/ml by using all hydrotropic agents in both methods. Correlation coefficients were found in the range of 0.9980 to 0.9999 for Method I and 0.9986 to 0.9999 for Method II respectively for estimation of Cefprozil in all the hydrotropic agents. The mean percentage recovery found for Cefprozil with all hydrotropic agents range from 97.19 to 100.93 % (method I) and 96.00 to 100.97 % (method II). The developed methods were found to be precise for estimation of Cefprozil by using all hydrotropic agents. The results of analysis obtained by the proposed method compared very well with those of USP standard limit. Area under curve method was found more sensitive than conventional Spectrophotometric method for estimation of Cefprozil. Solubility of Cefprozil was found more in urea and limit of detection and quantitation was found lower in potassium acetate as compared to other hydrotropic agents used in estimation.
|Julkaisu||International Journal of Pharmacy and Pharmaceutical Sciences|
|Tila||Julkaistu - tammik. 2010|
|OKM-julkaisutyyppi||A1 Julkaistu artikkeli, soviteltu|