Specific and innervation-regulated expression of the intermediate filament protein nestin at neuromuscular and myotendinous junctions in skeletal muscle

Vaittinen, Lukka, Cecilia Sahlgren, Rantanen, Hurme, Lendahl, John Eriksson, Kalimo

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    74 Sitaatiot (Scopus)

    Abstrakti

    The intermediate filament proteins nestin, vimentin, and desmin show a specific temporal expression pattern during the development of myofibers from myogenic precursor cells. Nestin and vimentin are actively expressed during early developmental stages to be later down-regulated, vimentin completely and nestin to minimal levels, whereas desmin expression begins later and is maintained in mature myofibers, in which desmin participates in maintaining structural integrity. In this study we have analyzed the expression levels and distribution pattern of nestin in intact and denervated muscle in rat and in human. Nestin immunoreactivity was specifically and focally localized in the sarcoplasm underneath neuromuscular junctions (NMJs) and in the vicinity of the myotendinous junctions (MTJs), ie, in regions associated with acetylcholine receptors (AChRs). This association prompted us to analyze nestin in neurogenically and myogenically denervated muscle. Immunoblot analysis disclosed a marked overall increase of accumulated nestin protein. Similar to the extrajunctional redistribution of AChRs in denervated myofibers, nestin immunoreactivity extended widely beyond the NMJ region. Re-innervation caused complete reversion of these changes. Our study demonstrates that the expression levels and distribution pattern of nestin are regulated by innervation, ie, signal transduction into myofibers.
    AlkuperäiskieliEi tiedossa
    Sivut591–600
    JulkaisuAmerican Journal of Pathology
    Vuosikerta154
    Numero2
    DOI - pysyväislinkit
    TilaJulkaistu - 1999
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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