Quantitative analysis of the self-assembly strategies of intermediate filaments from tetrameric vimentin

Eugen Czeizler, A Mizera, E Czeizler, Ralph-Johan Back, John Eriksson, Ion Petre

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    6 Sitaatiot (Scopus)

    Abstrakti

    In vitro assembly of intermediate filaments from tetrameric vimentin consists of a very rapid phase of tetramers laterally associating into unit-length filaments and a slow phase of filament elongation. We focus in this paper on a systematic quantitative investigation of two molecular models for filament assembly, recently proposed in (Kirmse et al. J. Biol. Chem. 282, 52 (2007), 18563-18572), through mathematical modeling, model fitting, and model validation. We analyze the quantitative contribution of each filament elongation strategy: with tetramers, with unit-length filaments, with longer filaments, or combinations thereof. In each case, we discuss the numerical fitting of the model with respect to one set of data, and its separate validation with respect to a second, different set of data. We introduce a high-resolution model for vimentin filament self-assembly, able to capture the detailed dynamics of filaments of arbitrary length. This provides much more predictive power for the model, in comparison to previous models where only the mean length of all filaments in the solution could be analyzed. We show how kinetic observations on low-resolution models can be extrapolated to the high-resolution model and used for lowering its complexity.
    AlkuperäiskieliEi tiedossa
    Sivut885–898
    JulkaisuIEEE/ACM Transactions on Computational Biology and Bioinformatics
    Vuosikerta9
    Numero3
    DOI - pysyväislinkit
    TilaJulkaistu - 2000
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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