Pre- and post-Golgi translocation of glucosylceramide in glycosphingolipid synthesis

D Halter, S Neumann, van Dijk SM, J Wolthoorn, De Maziere AM, OV Vieira, Peter Mattjus, J Klumperman, van Meer G, H Sprong

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    245 Sitaatiot (Scopus)

    Abstrakti

    Glycosphingolipids are controlled by the spatial organization of their metabolism and by trans port specificity. Using immunoelectron microscopy, we localize to the Golgi stack the glycosyltransferases that produce glucosylceramide (GlcCer), lactosylceramide (LacCer), and GM3. GlcCer is synthesized on the cytosolic side and must translocate across to the Golgi lumen for LacCer synthesis. However, only very little natural GlcCer translocates across the Golgi in vitro. As GlcCer reaches the cell surface when Golgi vesicular trafficking is inhibited, it must translocate across a post-Golgi membrane. Concanamycin, a vacuolar proton pump inhibitor, blocks translocation independently of multidrug transporters that are known to translocate short-chain GlcCer. Concanamycin did not reduce LacCer and GM3 synthesis. Thus, GlcCer destined for glycolipid synthesis follows a different pathway and transports back into the endoplasmic reticulum (ER) via the late Golgi protein FAPP2. FAPP2 knockdown strongly reduces GM3 synthesis. Overall, we show that newly synthesized GlcCer enters two pathways: one toward the noncytosolic surface of a post-Golgi membrane and one via the ER toward the Golgi lumen LacCer synthase.
    AlkuperäiskieliEi tiedossa
    Sivut101–115
    Sivumäärä115
    JulkaisuJournal of Cell Biology
    Vuosikerta179
    Numero1
    DOI - pysyväislinkit
    TilaJulkaistu - 2007
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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