TY - JOUR
T1 - Optimizing the performance of tin dioxide microspheres for phosphopeptide enrichment
AU - Leitner, Alexander
AU - Sturm, Martin
AU - Smått, Jan-Henrik
AU - Järn, Mikael
AU - Lindén, Mika
AU - Mechtler, Karl
AU - Lindner, Wolfgang
PY - 2009
Y1 - 2009
N2 - Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.
AB - Phosphopeptide enrichment based on metal oxide affinity chromatography is one of the most powerful tools for studying protein phosphorylation on a large scale. To complement existing metal oxide sorbents, we have recently introduced tin dioxide as a promising alternative. The preparation of SnO(2) microspheres by the nanocasting technique, using silica of different morphology as a template, offers a strategy to prepare materials that vary in their particle size and their porosity. Here, we demonstrate how such stannia materials can be successfully generated and their properties fine-tuned in order to obtain an optimized phosphopeptide enrichment material. We combined data from liquid chromatography-mass spectrometry experiments and physicochemical characterization, including nitrogen physisorption and energy-dispersive X-ray spectroscopy (EDX), to explain the influence of the various experimental parameters.
U2 - 10.1016/j.aca.2009.01.063
DO - 10.1016/j.aca.2009.01.063
M3 - Artikel
SN - 0003-2670
VL - 638
SP - 51
EP - 57
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 1
ER -