New crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatases.

P. Heikinheimo, Tiina Salminen, R. Lahti, B. Cooperman, A. Goldman

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

7 Sitaatiot (Scopus)

Abstrakti

We have obtained new crystal forms of Escherichia coli and Saccharomyces cerevisiae soluble inorganic pyrophosphatase with and without substrate, competitive inhibitor and divalent cation. They diffract to higher resolution than any forms previously reported. The best E. coli crystals are in space group R32 with cell dimensions of 111.4 x 111.4 x 76.6 Angstrom and diffract to 2.0 Angstrom. The best S. cerevisiae crystals were grown from a mixture of PEG 1000 and 4000 in the presence of metal ions. They are in space group P2(1)2(1)2(1), have cell dimensions of 54.2 x 68.5 x 161.7 Angstrom and diffract to 1.8 Angstrom.
AlkuperäiskieliEi tiedossa
Sivut399–401
Sivumäärä3
JulkaisuActa Crystallographica Section D: Biological Crystallography
Vuosikerta51
DOI - pysyväislinkit
TilaJulkaistu - 1995
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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