Myosin-X and talin modulate integrin activity at filopodia tips

Mitro Miihkinen, Max L.B. Grönloh, Ana Popović, Helena Vihinen, Eija Jokitalo, Benjamin T. Goult, Johanna Ivaska, Guillaume Jacquemet

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

28 Sitaatiot (Scopus)

Abstrakti

Filopodia assemble unique integrin-adhesion complexes to sense the extracellular matrix. However, the mechanisms of integrin regulation in filopodia are poorly defined. Here, we report that active integrins accumulate at the tip of myosin-X (MYO10)-positive filopodia, while inactive integrins are uniformly distributed. We identify talin and MYO10 as the principal integrin activators in filopodia. In addition, deletion of MYO10's FERM domain, or mutation of its β1-integrin-binding residues, reveals MYO10 as facilitating integrin activation, but not transport, in filopodia. However, MYO10's isolated FERM domain alone cannot activate integrins, potentially because of binding to both integrin tails. Finally, because a chimera construct generated by swapping MYO10-FERM by talin-FERM enables integrin activation in filopodia, our data indicate that an integrin-binding FERM domain coupled to a myosin motor is a core requirement for integrin activation in filopodia. Therefore, we propose a two-step integrin activation model in filopodia: receptor tethering by MYO10 followed by talin-mediated integrin activation.

AlkuperäiskieliEnglanti
Artikkeli109716
JulkaisuCell Reports
Vuosikerta36
Numero11
DOI - pysyväislinkit
TilaJulkaistu - 14 syysk. 2021
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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