Mitotic reorganization of the intermediate filament protein nestin involves phosphorylation by cdc2 kinase

Cecilia Sahlgren, Mikhailov, Hellman, Chou, Lendahl, Goldman, John Eriksson

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    99 Sitaatiot (Scopus)

    Abstrakti

    The intermediate filament protein nestin is expressed during early stages of development in the central nervous system and in muscle tissues. Nestin expression is associated with morphologically dynamic cells, such as dividing and migrating cells. However, little is known about regulation of nestin during these cellular processes. We have characterized the phosphorylation-based regulation of nestin during different stages of the cell cycle in a neuronal progenitor cell line, ST15A. Confocal microscopy of nestin organization and (32)P in vivo labeling studies show that the mitotic reorganization of nestin is accompanied by elevated phosphorylation of nestin. The phosphorylation-induced alterations in nestin organization during mitosis in ST15A cells are associated with partial disassembly of nestin filaments. Comparative in vitro and in vivo phosphorylation studies identified cdc2 as the primary mitotic kinase and Thr(316) as a cdc2-specific phosphorylation site on nestin. We generated a phosphospecific nestin antibody recognizing the phosphorylated form of this site. By using this antibody we observed that nestin shows constitutive phosphorylation at Thr(316), which is increased during mitosis. This study shows that nestin is reorganized during mitosis and that cdc2-mediated phosphorylation is an important regulator of nestin organization and dynamics during mitosis.
    AlkuperäiskieliEi tiedossa
    Sivut16456–16463
    JulkaisuJournal of Biological Chemistry
    Vuosikerta276
    Numero19
    DOI - pysyväislinkit
    TilaJulkaistu - 2001
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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