Investigation of protein binding with all solid-state ion-selective electrodes

Alok Prabhu, Johan Bobacka, Ari Ivaska, Kalle Levon

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    6 Sitaatiot (Scopus)

    Abstrakti

    A potentiometric sensor for studying charge based adsorption of proteins was created using a single-piece polyaniline-PVC ion-selective electrode (ISE). Three different ISEs, two for Na+ and one for Cl- ion determination, were studied. The Na+-ISEs consisted of a neutral calixarene-based ionophore and one with a charged carrier dinonylnapthalenesulfonic acid (DNNSA) whereas for the Cl- ISE, an anion exchanger tridodecylmethylammonium chloride (TDDMA(+)Cl(-)), was used. The Na+ ISE with DNNSA as the charged carrier was successfully able to discriminate the binding of two different proteins (bovine serum albumin and lysozyme) based on their intrinsic charge.
    AlkuperäiskieliEi tiedossa
    Sivut1887–1894
    Sivumäärä8
    JulkaisuElectroanalysis
    Vuosikerta25
    Numero8
    DOI - pysyväislinkit
    TilaJulkaistu - 2013
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

    Keywords

    • Conducting polymers
    • Electrochemical biosensors
    • Ion-selective electrodes
    • Potentiometry
    • Protein adsorption

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