In Vitro Characterization and Real-Time Label-Free Assessment of the Interaction of Chitosan-Coated Niosomes with Intestinal Cellular Monolayers

Elena Scurti; João Pedro Martins; Christian Celia; Paola Palumbo; Francesca Lombardi; Dalila Iannotta; Luisa Di Marzio; Hélder A. Santos; Tapani Viitala

doi:10.1021/acs.langmuir.3c00728

In Vitro Characterization and Real-Time Label-Free Assessment of the Interaction of Chitosan-Coated Niosomes with Intestinal Cellular Monolayers

Elena Scurti
, João Pedro Martins
, Christian Celia
, Paola Palumbo
, Francesca Lombardi
, Dalila Iannotta
, Luisa Di Marzio^*
, Hélder A. Santos^*
, Tapani Viitala^*

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Lehtiartikkeli › Artikkeli › Tieteellinen › vertaisarvioitu

9 Sitaatiot (Scopus)

115 Lataukset (Pure)

Abstrakti

In vitro cell-based characterization methods of nanoparticles are generally static and require the use of secondary analysis techniques and labeling agents. In this study, bare niosomes and chitosan-coated niosomes (chitosomes) and their interactions with intestinal cells are studied under dynamic conditions and without fluorescent probes, using surface plasmon resonance (SPR)-based cell sensing. Niosomes and chitosomes were synthesized by using Tween 20 and cholesterol in a 15 mM:15 mM ratio and then characterized by dynamic light scattering (DLS). DLS analysis demonstrated that bare niosomes had average sizes of ∼125 nm, polydispersity index (PDI) below 0.2, and a negative zeta (ζ)-potential of −35.6 mV. In turn, chitosomes had increased sizes up to ∼180 nm, with a PDI of 0.2-0.3 and a highly positive ζ-potential of +57.9 mV. The viability of HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultured cells showed that both niosomes and chitosomes are cytocompatible up to concentrations of 31.6 μg/mL for at least 240 min. SPR analysis demonstrated that chitosomes interact more efficiently with HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultures compared to bare niosomes. The resulting SPR measurements were further supported by confocal microscopy and flow cytometry studies, which demonstrated that this method is a useful complementary or even alternative tool to directly characterize the interactions between niosomes and in vitro cell models in label-free and real-time conditions.

Alkuperäiskieli	Englanti
Sivut	8255-8266
Sivumäärä	12
Julkaisu	Langmuir
Vuosikerta	39
Numero	23
DOI - pysyväislinkit	https://doi.org/10.1021/acs.langmuir.3c00728
Tila	Julkaistu - 13 kesäk. 2023
OKM-julkaisutyyppi	A1 Julkaistu artikkeli, soviteltu

Rahoitus

This manuscript was partially supported by Ministero dell’Istruzione, dell’Università e della Ricerca [FAR 2017, FAR 2018 (D56C18000780005), FAR 2019 (D54I19002790005)] to C.C. and L.D.M.; the Italian Ministry of Education, University and Research, Italy, under the national project Programma Operativo Nazionale Ricerca e Innovazione (PON) 2014-2020 (CCI 2014IT16M2OP005) Fondo Sociale Europeo, Azione I.1, Dottorati Innovativi con Caratterizzazione Industriale to D.I. H.A.S. acknowledges financial support from the Academy of Finland (No. 331151), the Sigrid Jusélius Foundation, and the UMCG Research Funds. T.V. and E.S. acknowledge financial support from the Academy of Finland (No. 13241774). E.S. also acknowledges the financial support of the Erasmus+ programme of the European Union. The authors acknowledge the following core facilities funded by Biocenter Finland: Electron Microscopy Unity of the University of Helsinki for providing the facilities for TEM imaging and the Light Microscopy Unit of the Institute of Biotechnology for the confocal microscope.

Pääsy asiakirjaan

10.1021/acs.langmuir.3c00728

acs.langmuir.3c00728Lopullinen julkaistu versio, 6,99 MBLisenssi: CC BY

https://urn.fi/URN:NBN:fi-fe20230905117408

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@article{ea7e88d6988b44509864e4933c80f785,

title = "In Vitro Characterization and Real-Time Label-Free Assessment of the Interaction of Chitosan-Coated Niosomes with Intestinal Cellular Monolayers",

abstract = "In vitro cell-based characterization methods of nanoparticles are generally static and require the use of secondary analysis techniques and labeling agents. In this study, bare niosomes and chitosan-coated niosomes (chitosomes) and their interactions with intestinal cells are studied under dynamic conditions and without fluorescent probes, using surface plasmon resonance (SPR)-based cell sensing. Niosomes and chitosomes were synthesized by using Tween 20 and cholesterol in a 15 mM:15 mM ratio and then characterized by dynamic light scattering (DLS). DLS analysis demonstrated that bare niosomes had average sizes of ∼125 nm, polydispersity index (PDI) below 0.2, and a negative zeta (ζ)-potential of −35.6 mV. In turn, chitosomes had increased sizes up to ∼180 nm, with a PDI of 0.2-0.3 and a highly positive ζ-potential of +57.9 mV. The viability of HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultured cells showed that both niosomes and chitosomes are cytocompatible up to concentrations of 31.6 μg/mL for at least 240 min. SPR analysis demonstrated that chitosomes interact more efficiently with HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultures compared to bare niosomes. The resulting SPR measurements were further supported by confocal microscopy and flow cytometry studies, which demonstrated that this method is a useful complementary or even alternative tool to directly characterize the interactions between niosomes and in vitro cell models in label-free and real-time conditions.",

author = "Elena Scurti and Martins, \{Jo{\~a}o Pedro\} and Christian Celia and Paola Palumbo and Francesca Lombardi and Dalila Iannotta and \{Di Marzio\}, Luisa and Santos, \{H{\'e}lder A.\} and Tapani Viitala",

note = "Funding Information: This manuscript was partially supported by Ministero dell{\textquoteright}Istruzione, dell{\textquoteright}Universit{\`a} e della Ricerca [FAR 2017, FAR 2018 (D56C18000780005), FAR 2019 (D54I19002790005)] to C.C. and L.D.M.; the Italian Ministry of Education, University and Research, Italy, under the national project Programma Operativo Nazionale Ricerca e Innovazione (PON) 2014-2020 (CCI 2014IT16M2OP005) Fondo Sociale Europeo, Azione I.1, Dottorati Innovativi con Caratterizzazione Industriale to D.I. H.A.S. acknowledges financial support from the Academy of Finland (No. 331151), the Sigrid Jus{\'e}lius Foundation, and the UMCG Research Funds. T.V. and E.S. acknowledge financial support from the Academy of Finland (No. 13241774). E.S. also acknowledges the financial support of the Erasmus+ programme of the European Union. The authors acknowledge the following core facilities funded by Biocenter Finland: Electron Microscopy Unity of the University of Helsinki for providing the facilities for TEM imaging and the Light Microscopy Unit of the Institute of Biotechnology for the confocal microscope. Publisher Copyright: {\textcopyright} 2023 The Authors. Published by American Chemical Society.",

year = "2023",

month = jun,

day = "13",

doi = "10.1021/acs.langmuir.3c00728",

language = "English",

volume = "39",

pages = "8255--8266",

journal = "Langmuir",

issn = "0743-7463",

publisher = "American chemical society",

number = "23",

}

TY - JOUR

T1 - In Vitro Characterization and Real-Time Label-Free Assessment of the Interaction of Chitosan-Coated Niosomes with Intestinal Cellular Monolayers

AU - Scurti, Elena

AU - Martins, João Pedro

AU - Celia, Christian

AU - Palumbo, Paola

AU - Lombardi, Francesca

AU - Iannotta, Dalila

AU - Di Marzio, Luisa

AU - Santos, Hélder A.

AU - Viitala, Tapani

N1 - Funding Information: This manuscript was partially supported by Ministero dell’Istruzione, dell’Università e della Ricerca [FAR 2017, FAR 2018 (D56C18000780005), FAR 2019 (D54I19002790005)] to C.C. and L.D.M.; the Italian Ministry of Education, University and Research, Italy, under the national project Programma Operativo Nazionale Ricerca e Innovazione (PON) 2014-2020 (CCI 2014IT16M2OP005) Fondo Sociale Europeo, Azione I.1, Dottorati Innovativi con Caratterizzazione Industriale to D.I. H.A.S. acknowledges financial support from the Academy of Finland (No. 331151), the Sigrid Jusélius Foundation, and the UMCG Research Funds. T.V. and E.S. acknowledge financial support from the Academy of Finland (No. 13241774). E.S. also acknowledges the financial support of the Erasmus+ programme of the European Union. The authors acknowledge the following core facilities funded by Biocenter Finland: Electron Microscopy Unity of the University of Helsinki for providing the facilities for TEM imaging and the Light Microscopy Unit of the Institute of Biotechnology for the confocal microscope. Publisher Copyright: © 2023 The Authors. Published by American Chemical Society.

PY - 2023/6/13

Y1 - 2023/6/13

N2 - In vitro cell-based characterization methods of nanoparticles are generally static and require the use of secondary analysis techniques and labeling agents. In this study, bare niosomes and chitosan-coated niosomes (chitosomes) and their interactions with intestinal cells are studied under dynamic conditions and without fluorescent probes, using surface plasmon resonance (SPR)-based cell sensing. Niosomes and chitosomes were synthesized by using Tween 20 and cholesterol in a 15 mM:15 mM ratio and then characterized by dynamic light scattering (DLS). DLS analysis demonstrated that bare niosomes had average sizes of ∼125 nm, polydispersity index (PDI) below 0.2, and a negative zeta (ζ)-potential of −35.6 mV. In turn, chitosomes had increased sizes up to ∼180 nm, with a PDI of 0.2-0.3 and a highly positive ζ-potential of +57.9 mV. The viability of HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultured cells showed that both niosomes and chitosomes are cytocompatible up to concentrations of 31.6 μg/mL for at least 240 min. SPR analysis demonstrated that chitosomes interact more efficiently with HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultures compared to bare niosomes. The resulting SPR measurements were further supported by confocal microscopy and flow cytometry studies, which demonstrated that this method is a useful complementary or even alternative tool to directly characterize the interactions between niosomes and in vitro cell models in label-free and real-time conditions.

AB - In vitro cell-based characterization methods of nanoparticles are generally static and require the use of secondary analysis techniques and labeling agents. In this study, bare niosomes and chitosan-coated niosomes (chitosomes) and their interactions with intestinal cells are studied under dynamic conditions and without fluorescent probes, using surface plasmon resonance (SPR)-based cell sensing. Niosomes and chitosomes were synthesized by using Tween 20 and cholesterol in a 15 mM:15 mM ratio and then characterized by dynamic light scattering (DLS). DLS analysis demonstrated that bare niosomes had average sizes of ∼125 nm, polydispersity index (PDI) below 0.2, and a negative zeta (ζ)-potential of −35.6 mV. In turn, chitosomes had increased sizes up to ∼180 nm, with a PDI of 0.2-0.3 and a highly positive ζ-potential of +57.9 mV. The viability of HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultured cells showed that both niosomes and chitosomes are cytocompatible up to concentrations of 31.6 μg/mL for at least 240 min. SPR analysis demonstrated that chitosomes interact more efficiently with HT29-MTX, Caco-2, and Caco-2/HT29-MTX cocultures compared to bare niosomes. The resulting SPR measurements were further supported by confocal microscopy and flow cytometry studies, which demonstrated that this method is a useful complementary or even alternative tool to directly characterize the interactions between niosomes and in vitro cell models in label-free and real-time conditions.

UR - https://www.scopus.com/pages/publications/85163265697

U2 - 10.1021/acs.langmuir.3c00728

DO - 10.1021/acs.langmuir.3c00728

M3 - Article

C2 - 37265082

AN - SCOPUS:85163265697

SN - 0743-7463

VL - 39

SP - 8255

EP - 8266

JO - Langmuir

JF - Langmuir

IS - 23

ER -

In Vitro Characterization and Real-Time Label-Free Assessment of the Interaction of Chitosan-Coated Niosomes with Intestinal Cellular Monolayers

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