High-resolution live imaging of tardigrade response to anoxia

Anna Mari Haapanen-Saaristo; Sara Calhim; Ilkka Paatero

doi:10.1016/j.micron.2025.103847

High-resolution live imaging of tardigrade response to anoxia

Anna Mari Haapanen-Saaristo
, Sara Calhim
, Ilkka Paatero^*

^*Tämän työn vastaava kirjoittaja

Turun biotiedekeskus

Tutkimustuotos: Lehtiartikkeli › Artikkeli › Tieteellinen › vertaisarvioitu

1 Sitaatiot (Scopus)

3 Lataukset (Pure)

Abstrakti

Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen – anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.

Alkuperäiskieli	Englanti
Artikkeli	103847
Julkaisu	Micron
Vuosikerta	196-197
DOI - pysyväislinkit	https://doi.org/10.1016/j.micron.2025.103847
Tila	Julkaistu - syysk. 2025
OKM-julkaisutyyppi	A1 Julkaistu artikkeli, soviteltu

Rahoitus

The authors declare the following financial interests/personal relationships which may be considered as potential competing interests: Ilkka Paatero reports financial support was provided by Business Finland. Ilkka Paatero reports financial support was provided by Finnish Foundation for Cardiovascular Research. University of Turku has registered trademark 3DFLUOHISTO®, and I.P. is involved in the commercialization of 3DFLUOHISTO-technology. Other authors declare that they have no conflict of interest. If there are other authors, they declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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10.1016/j.micron.2025.103847Lisenssi: CC BY

1-s2.0-S0968432825000654-mainLopullinen julkaistu versio, 4,81 MBLisenssi: CC BY

https://urn.fi/URN:NBN:fi-fe20251205114791

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title = "High-resolution live imaging of tardigrade response to anoxia",

abstract = "Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen – anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.",

keywords = "anoxybiosis, Fluorescence microscopy, hypoxia, intravital imaging, tardigrade",

author = "Haapanen-Saaristo, \{Anna Mari\} and Sara Calhim and Ilkka Paatero",

note = "Publisher Copyright: {\textcopyright} 2025",

year = "2025",

month = sep,

doi = "10.1016/j.micron.2025.103847",

language = "English",

volume = "196-197",

journal = "Micron",

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TY - JOUR

T1 - High-resolution live imaging of tardigrade response to anoxia

AU - Haapanen-Saaristo, Anna Mari

AU - Calhim, Sara

AU - Paatero, Ilkka

PY - 2025/9

Y1 - 2025/9

N2 - Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen – anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.

AB - Tardigrades are well-known for their ability to tolerate extreme environmental conditions such as heat, drought and lack of oxygen by undergoing cryptobiosis. The molecular responses to stress have been studied in detail, but the physiological and morphogenetic changes during cryptobiosis are less understood. We developed new live high-resolution fluorescence microscopy protocols to visualize the tardigrade response to lack of oxygen – anoxybiosis. High-resolution time-lapse imaging enabled analysis of cellular morphology and tracking of cell movements during anoxybiosis. These analyses revealed considerable changes in morphology, composition and movement of storage cells. Our observations and new imaging protocols can be used to study morphological and cellular response to stress in tardigrades.

KW - anoxybiosis

KW - Fluorescence microscopy

KW - hypoxia

KW - intravital imaging

KW - tardigrade

UR - https://www.scopus.com/pages/publications/105004871891

U2 - 10.1016/j.micron.2025.103847

DO - 10.1016/j.micron.2025.103847

M3 - Article

C2 - 40367672

AN - SCOPUS:105004871891

SN - 0968-4328

VL - 196-197

JO - Micron

JF - Micron

M1 - 103847

ER -

High-resolution live imaging of tardigrade response to anoxia

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