Glycosylphosphatidylinositol (GPI)-anchoring of mamba toxins enables cell-restricted receptor silencing

Katja Näreoja, Lauri M. Louhivuori, Karl E.O. Åkerman, Jussi Meriluoto, Johnny Näsman*

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

1 Sitaatiot (Scopus)

Abstrakti

Muscarinic toxins (MTs) are snake venom peptides found to selectively target specific subtypes of G-protein-coupled receptors. In here, we have attached a glycosylphosphatidylinositol (GPI) tail to three different toxin molecules and evaluated their receptor-blocking effects in a heterologous expression system. MT7-GPI remained anchored to the cell surface and selectively inhibited M 1 muscarinic receptor signaling expressed in the same cell. To further demonstrate the utility of the GPI tail, we generated MT3- and MTα-like gene sequences and fused these to the signal sequence for GPI attachment. Functional assessment of these membrane-anchored toxins on coexpressed target receptors indicated a prominent antagonistic effect. In ligand binding experiments the GPI-anchored toxins were found to exhibit similar selection profiles among receptor subtypes as the soluble toxins. The results indicate that GPI attachment of MTs and related receptor toxins could be used to assess the role of receptor subtypes in specific organs or even cells in vivo by transgenic approaches.

AlkuperäiskieliEnglanti
Sivut93-97
Sivumäärä5
JulkaisuBiochemical and Biophysical Research Communications
Vuosikerta417
Numero1
DOI - pysyväislinkit
TilaJulkaistu - 6 tammikuuta 2012
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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