Fluorescent single-digit detonation nanodiamond for biomedical applications

N Nunn, M d’Amora, Neeraj Prabhakar, AM Panich, N Froumin, MD Torelli, I Vlasov, P Reineck, B Gibson, Jessica Rosenholm, S Giordani, O Shenderova

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

22 Sitaatiot (Scopus)

Abstrakti

Detonation nanodiamonds (DNDs) have emerged as promising candidates for a variety of biomedical applications, thanks to different physicochemical and biological properties, such as small size and reactive surfaces. In this study, we propose carbon dot decorated single digit (4–5 nm diameter) primary particles of detonation nanodiamond as promising fluorescent probes. Due to their intrinsic fluorescence originating from tiny (1–2 atomic layer thickness) carbonaceous structures on their surfaces, they exhibit brightness suitable for in vitro imaging. Moreover, this material offers a unique, cost effective alternative to sub-10 nm nanodiamonds containing fluorescent nitrogen-vacancy color centers, which have not yet been produced at large scale. In this paper, carbon dot decorated nanodiamonds are characterized by several analytical techniques. In addition, the efficient cellular uptake and fluorescence of these particles are observed in vitro on MDA-MD-231 breast cancer cells by means of confocal imaging. Finally, the in vivo biocompatibility of carbon dot decorated nanodiamonds is demonstrated in zebrafish during the development. Our results indicate the potential of single-digit detonation nanodiamonds as biocompatible fluorescent probes. This unique material will find application in correlative light and electron microscopy, where small sized NDs can be attached to antibodies to act as a suitable dual marker for intracellular correlative microscopy of biomolecules.

AlkuperäiskieliEi tiedossa
Sivut035010–
JulkaisuMethods and Applications in Fluorescence
Vuosikerta6
Numero3
DOI - pysyväislinkit
TilaJulkaistu - 2018
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

Keywords

  • nanodiamonds
  • bioimaging
  • fluorescence microscopy

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