Enhanced antibody responses in CD19-Cre mice

Diogo M. Cunha; Sara Hernández-Pérez; Pieta K. Mattila

doi:10.1038/s41598-024-83954-8

Enhanced antibody responses in CD19-Cre mice

Diogo M. Cunha
, Sara Hernández-Pérez
, Pieta K. Mattila^*

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Lehtiartikkeli › Artikkeli › Tieteellinen › vertaisarvioitu

2 Sitaatiot (Scopus)

31 Lataukset (Pure)

Abstrakti

CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

Alkuperäiskieli	Englanti
Artikkeli	1348
Julkaisu	Scientific Reports
Vuosikerta	15
Numero	1
DOI - pysyväislinkit	https://doi.org/10.1038/s41598-024-83954-8
Tila	Julkaistu - 8 tammik. 2025
OKM-julkaisutyyppi	A1 Julkaistu artikkeli, soviteltu

Rahoitus

We are thankful to Laura Grönfors, M. Özge Balci and Chiara Urban for technical assistance. Turku Bioscience Cell Imaging and Cytometry Core and Biocenter Finland are acknowledged for providing research infrastructures. This work was supported by the Research Council of Finland’s Flagship InFLAMES (decision numbers: 337530 and 357910) and project funding (decision numbers: 296684, 327378, and 339810 to P.K.M.), and the Sigrid Jusélius (to P.K.M.) and Finnish Cultural Foundations (D.M.C. and S.H-P).

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10.1038/s41598-024-83954-8

s41598-024-83954-8Lopullinen julkaistu versio, 3,2 MBLisenssi: CC BY-NC-ND

https://urn.fi/URN:NBN:fi-fe20251128112490

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title = "Enhanced antibody responses in CD19-Cre mice",

abstract = "CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.",

keywords = "Antibody response, B cells, BCR, CD19, Class-switch recombination",

author = "Cunha, \{Diogo M.\} and Sara Hern{\'a}ndez-P{\'e}rez and Mattila, \{Pieta K.\}",

note = "Publisher Copyright: {\textcopyright} The Author(s) 2025.",

year = "2025",

month = jan,

day = "8",

doi = "10.1038/s41598-024-83954-8",

language = "English",

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TY - JOUR

T1 - Enhanced antibody responses in CD19-Cre mice

AU - Cunha, Diogo M.

AU - Hernández-Pérez, Sara

AU - Mattila, Pieta K.

N1 - Publisher Copyright: © The Author(s) 2025.

PY - 2025/1/8

Y1 - 2025/1/8

N2 - CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

AB - CD19-Cre is an important and widely used Cre-lox model for B cell-specific genetic manipulation in murine systems. Mice carrying one allele of CD19-Cre are, at the same time, rendered heterozygote for CD19, a crucial coreceptor of the B cell antigen receptor (BCR). As a result, CD19-Cre mice exhibit diminished expression levels of CD19, with potential, yet insufficiently examined, consequences in B cell activation. Here, we report significantly heightened antibody responses upon both T-dependent (NP-KLH) and T-independent (NP-Ficoll) immunizations as well as elevated levels of basal IgM immunoglobulin levels in CD19-Cre mice. In vitro, we observed enhanced class-switch recombination and a moderate reduction in B cell proliferation upon LPS and IFNγ stimulation, yet no drastic differences in BCR signalling. Our findings warrant careful consideration in the use of CD19-Cre mouse model in B cell research.

KW - Antibody response

KW - B cells

KW - BCR

KW - CD19

KW - Class-switch recombination

UR - https://www.scopus.com/pages/publications/85215066456

U2 - 10.1038/s41598-024-83954-8

DO - 10.1038/s41598-024-83954-8

M3 - Article

C2 - 39779739

AN - SCOPUS:85215066456

SN - 2045-2322

VL - 15

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 1348

ER -

Enhanced antibody responses in CD19-Cre mice

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