TY - JOUR
T1 - Effect of laminin, polylysine and cell medium components on the attachment of human hepatocellular carcinoma cells to cellulose nanofibrils analyzed by surface plasmon resonance
AU - Zhang, Xue
AU - Viitala, Tapani
AU - Harjumäki, Riina
AU - Kartal-Hodzic, Alma
AU - Valle-Delgado, Juan José
AU - Österberg, Monika
N1 - Funding Information:
The authors thank the Academy of Finland for the financial support for this work (Project Number 278279 , MIMEGEL), and Mauri Kostiainen and his group for the use of cell culture facilities at Aalto University. Xue Zhang thanks Dr. Apeksha Damania for helping with cell cultures. Xue Zhang is grateful for the financial support from both the FinnCERES Materials Bioeconomy Ecosystem and Puunjalostusinsinöörit ry, PI while preparing the manuscript. This work made use of Aalto University Bioeconomy Facilities.
Publisher Copyright:
© 2020 The Authors
PY - 2021/2/15
Y1 - 2021/2/15
N2 - The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. Plant-based, chemically unmodified cellulose nanofibrils (CNF) hydrogel is a natural, abundant, and biocompatible material that has attracted great attention for biomedical applications, in particular for three-dimensional cell cultures. However, the mechanisms of cell-CNF interactions and factors that affect these interactions are not yet fully understood. In this work, multi-parametric surface plasmon resonance (SPR) was used to study how the adsorption of human hepatocellular carcinoma (HepG2) cells on CNF films is affected by the different proteins and components of the cell medium. Both human recombinant laminin-521 (LN-521, a natural protein of the extracellular matrix) and poly-L-lysine (PLL) adsorbed on CNF films and enhanced the attachment of HepG2 cells. Cell medium components (glucose and amino acids) and serum proteins (fetal bovine serum, FBS) also adsorbed on both bare CNF and on protein-coated CNF substrates. However, the adsorption of FBS hindered the attachment of HepG2 cells to LN-521- and PLL-coated CNF substrates, suggesting that serum proteins blocked the formation of laminin-integrin bonds and decreased favorable PLL-cell electrostatic interactions. This work sheds light on the effect of different factors on cell attachment to CNF, paving the way for the utilization and optimization of CNF-based materials for different tissue engineering applications.
AB - The development of in vitro cell models that mimic cell behavior in organs and tissues is an approach that may have remarkable impact on drug testing and tissue engineering applications in the future. Plant-based, chemically unmodified cellulose nanofibrils (CNF) hydrogel is a natural, abundant, and biocompatible material that has attracted great attention for biomedical applications, in particular for three-dimensional cell cultures. However, the mechanisms of cell-CNF interactions and factors that affect these interactions are not yet fully understood. In this work, multi-parametric surface plasmon resonance (SPR) was used to study how the adsorption of human hepatocellular carcinoma (HepG2) cells on CNF films is affected by the different proteins and components of the cell medium. Both human recombinant laminin-521 (LN-521, a natural protein of the extracellular matrix) and poly-L-lysine (PLL) adsorbed on CNF films and enhanced the attachment of HepG2 cells. Cell medium components (glucose and amino acids) and serum proteins (fetal bovine serum, FBS) also adsorbed on both bare CNF and on protein-coated CNF substrates. However, the adsorption of FBS hindered the attachment of HepG2 cells to LN-521- and PLL-coated CNF substrates, suggesting that serum proteins blocked the formation of laminin-integrin bonds and decreased favorable PLL-cell electrostatic interactions. This work sheds light on the effect of different factors on cell attachment to CNF, paving the way for the utilization and optimization of CNF-based materials for different tissue engineering applications.
KW - Cell adsorption
KW - Cellulose nanofibrils
KW - Interactions
KW - Proteins
KW - Surface plasmon resonance
UR - http://www.scopus.com/inward/record.url?scp=85092493886&partnerID=8YFLogxK
U2 - 10.1016/j.jcis.2020.09.080
DO - 10.1016/j.jcis.2020.09.080
M3 - Article
C2 - 33069029
AN - SCOPUS:85092493886
SN - 0021-9797
VL - 584
SP - 310
EP - 319
JO - Journal of Colloid and Interface Science
JF - Journal of Colloid and Interface Science
ER -