Effect of bioactive glass air‐abrasion on Fusobacterium nucleatum and Porphyromonas gingivalis biofilm formed on moderately rough titanium surface

This aim of this study was to investigate the effects of three types of air-abrasion particles on dual-species biofilms of Fusobacterium nucleatum and Porphyromonas gingivalis, both of which were cultured on sandblasted and acid-etched (SA) titanium discs. Out of 24 SA discs with biofilm, 18 were exposed to either air-abrasion using Bioglass 45S5 (45S5 BG; n = 6), novel zinc (Zn)-containing bioactive glass (Zn4 BG; n = 6), or inert glass (n = 6). The efficiency of biofilm removal was evaluated using scanning electron microscopy (SEM) imaging and culturing techniques. Air-abrasion using 45S5 BG or Zn4 BG demonstrated a significant decrease in the total number of viable bacteria compared to discs air-abraded with inert glass or intact biofilm without abrasion. Moreover, P. gingivalis could not be detected from SEM images nor culture plates after air-abrasion with 45S5 BG or Zn4 BG. The present study showed that air-abrasion with 45S5 or Zn4 bioactive glasses can successfully eradicate dual-biofilm of F. nucleatum and P. gingivalis from sandblasted and acid-etched titanium discs.