Diatom-inspired skeletonisation of insulin - mechanistic insights into crystallisation and extracellular bioactivity

Diosangeles Soto Véliz, Catharina Alam, Thiago Neitzel, Rebecca Wyborski, Adolfo Rivero-Müller, Parvez Alam

    Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

    2 Sitaatiot (Scopus)

    Abstrakti

    In this paper, we encage insulin within calcium carbonate by means of a biomineralisation process. We find that both dogbone and crossbone morphologies develop during the crystallisation process. The crystals break down into small nanocrystals after prolonged immersion in phosphate buffer solution, which adhere extracellularly to mammalian cells without causing any observable damage or early cell-death. The mechanisms behind calcium carbonate encaging of single insulin monomers are detailed. This communication elucidates a novel, diatom-inspired approach to the mineral skeletonisation of insulin.
    AlkuperäiskieliEi tiedossa
    Sivut140–147
    JulkaisuColloids and Surfaces B: Biointerfaces
    Vuosikerta133
    DOI - pysyväislinkit
    TilaJulkaistu - 2015
    OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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