Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

Milla Riina Neffling; Emilie Lance; Jussi Meriluoto

doi:10.1016/j.envpol.2009.10.023

Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

Milla Riina Neffling^*
, Emilie Lance
, Jussi Meriluoto

^*Tämän työn vastaava kirjoittaja

Tutkimustuotos: Lehtiartikkeli › Artikkeli › Tieteellinen › vertaisarvioitu

72 Sitaatiot (Scopus)

Abstrakti

Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

Alkuperäiskieli	Englanti
Sivut	948-952
Sivumäärä	5
Julkaisu	Environmental Pollution
Vuosikerta	158
Numero	3
DOI - pysyväislinkit	https://doi.org/10.1016/j.envpol.2009.10.023
Tila	Julkaistu - maalisk. 2010
OKM-julkaisutyyppi	A1 Julkaistu artikkeli, soviteltu

Rahoitus

The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska understödsföreningen Liv och Hälsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study.

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10.1016/j.envpol.2009.10.023

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@article{fc6649457b62463391ce3d47b9d6801c,

title = "Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry",

abstract = "Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40\%), the extraction efficiency good (62-97\%), but the signal suppression effect from the matrix remained severe in our system (16-37\% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100\%, depending on the sample and the toxin variant and concentration.",

keywords = "Microcystins, MMPB method, Total contamination levels",

author = "Neffling, \{Milla Riina\} and Emilie Lance and Jussi Meriluoto",

note = "Funding Information: The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska underst{\"o}dsf{\"o}reningen Liv och H{\"a}lsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.",

year = "2010",

month = mar,

doi = "10.1016/j.envpol.2009.10.023",

language = "English",

volume = "158",

pages = "948--952",

journal = "Environmental Pollution",

issn = "0269-7491",

publisher = "Elsevier",

number = "3",

}

TY - JOUR

T1 - Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

AU - Neffling, Milla Riina

AU - Lance, Emilie

AU - Meriluoto, Jussi

N1 - Funding Information: The authors would like to thank the National Graduate school in Informational and Structural Biology, Medicinska understödsföreningen Liv och Hälsa, Otto A. Malms donations fond (M-R N.), and the CIMO (Center for International Mobility) and the French and Finnish Ministries of Foreign Affairs (E.L.), Tekes decision number 40366/06 and Academy of Finland decision number 108947 (J.M.). Dr. Krister Karlsson is thanked for his helpful advice in the early stages of the study. Copyright: Copyright 2010 Elsevier B.V., All rights reserved.

PY - 2010/3

Y1 - 2010/3

N2 - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

AB - Microcystins are cyanobacterial hepatotoxins capable of accumulation into animal tissues. The toxins act by inhibiting specific protein phosphatases and both non-covalent and covalent interactions occur. The 2-methyl-3-methoxy-4-phenylbutyric acid (MMPB) method determines the total, i.e. the sum of free and protein-bound microcystin in tissues. The aim of the method development in this paper was to tackle the problems with the MMPB methodology: the rather laborious workflow and the loss of material during different steps of the method. In the optimised workflow the oxidation recovery was of acceptable level (29-40%), the extraction efficiency good (62-97%), but the signal suppression effect from the matrix remained severe in our system (16-37% signal left). The extraction efficiency for the determination of the free, extractable microcystins, was found to be good, 52-100%, depending on the sample and the toxin variant and concentration.

KW - Microcystins

KW - MMPB method

KW - Total contamination levels

UR - https://www.scopus.com/pages/publications/74149091840

U2 - 10.1016/j.envpol.2009.10.023

DO - 10.1016/j.envpol.2009.10.023

M3 - Article

C2 - 19910094

AN - SCOPUS:74149091840

SN - 0269-7491

VL - 158

SP - 948

EP - 952

JO - Environmental Pollution

JF - Environmental Pollution

IS - 3

ER -

Detection of free and covalently bound microcystins in animal tissues by liquid chromatography-tandem mass spectrometry

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