Analysis of the plasma proteome using iTRAQ and TMT-based Isobaric labeling

R Moulder, SD Bhosale, DR Goodlett, Riitta Lahesmaa

Tutkimustuotos: LehtiartikkeliKatsausartikkeliTieteellinenvertaisarvioitu

64 Sitaatiot (Scopus)

Abstrakti

Over the past decade, chemical labeling with isobaric tandem mass tags, such as isobaric tags for relative and absolute quantification reagents (iTRAQ) and tandem mass tag (TMT) reagents, has been employed in a wide range of different clinically orientated serum and plasma proteomics studies. In this review the scope of these works is presented with attention to the areas of research, methods employed and performance limitations. These applications have covered a wide range of diseases, disorders and infections, and have implemented a variety of different preparative and mass spectrometric approaches. In contrast to earlier works, which struggled to quantify more than a few hundred proteins, increasingly these studies have provided deeper insight into the plasma proteome extending the numbers of quantified proteins to over a thousand.
AlkuperäiskieliEi tiedossa
Sivut583–606
Sivumäärä24
JulkaisuMass Spectrometry Reviews
Vuosikerta37
Numero5
DOI - pysyväislinkit
TilaJulkaistu - 2018
OKM-julkaisutyyppiA2 Arvio tiedejulkaisuussa (artikkeli)

Keywords

  • disease research
  • isobaric labeling
  • immunodepletion
  • peptide fractionation
  • protein biomarkers
  • plasma

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