A solid-liquid extraction and high performance thin layer chromatographic determination of diacerein and aceclofenac in pharmaceutical tablet dosage form

Santosh Bhalerao, Santosh Tambe*, Vikas Pareek, Rupali Shinde, Lalit Kumar Gupta

*Tämän työn vastaava kirjoittaja

Tutkimustuotos: LehtiartikkeliArtikkeliTieteellinenvertaisarvioitu

11 Sitaatiot (Scopus)

Abstrakti

A novel, simple and robust High Performance Thin Layer Chromatographic method was developed and validated for the determination of diacerein and aceclofenac in the combined pharmaceutical tablet dosage form. The developed method demonstrates extraction of diacerein and aceclofenac by solid - liquid extraction and densitometric determination of them. Paracetamol was used as an internal standard (IS). The precoated silica gel 60F254 aluminum plate was selected as the stationary phase and the mixture of ethyl acetate: methanol: glacial acetic acid in the ratio of (12: 0.5: 0.2 v/v/v) was used as developing solvents. The detection of diacerein and aceclofenac was carried out at 268 nm by TLC scanner-3(Camag). The developed method was validated for linearity, accuracy, precision, limit of detection, limit of quantitation and robustness parameters. The correlation coefficient of diacerein and aceclofenac were 0.9997 and 0.9996 observed respectively. The average percentage recovery of diacerein and aceclofenac were found to be 100.14 ± 1.15 and 100.71 ± 0.33 respectively. Intra and inter day precision measured as coefficient of variation were less than 2% for both analytes. The proposed HPTLC method has potential applications for determination of diacerein and aceclofenac in combined tablet dosage form.

AlkuperäiskieliEnglanti
Sivut25-30
Sivumäärä6
JulkaisuAsian Journal of Pharmaceutical and Clinical Research
Vuosikerta3
Numero1
TilaJulkaistu - tammik. 2010
Julkaistu ulkoisestiKyllä
OKM-julkaisutyyppiA1 Julkaistu artikkeli, soviteltu

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