Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors

Angelo Tricase; Anna Imbriano; Marlene Valentino; Nicoletta Ditaranto; Eleonora Macchia; Cinzia Di Franco; Reshma Kidayaveettil; Dónal Leech; Matteo Piscitelli; Gaetano Scamarcio; Gaetano Perchiazzi; Luisa Torsi; Paolo Bollella

doi:10.1002/adsr.202300036

Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors

Angelo Tricase
, Anna Imbriano
, Marlene Valentino
, Nicoletta Ditaranto
, Eleonora Macchia
, Cinzia Di Franco
, Reshma Kidayaveettil
, Dónal Leech
, Matteo Piscitelli
, Gaetano Scamarcio
, Gaetano Perchiazzi
, Luisa Torsi
, Paolo Bollella

Physics

Research output: Contribution to journal › Article › Scientific › peer-review

19 Citations (Scopus)

25 Downloads (Pure)

Abstract

Herein, this work reports the first example of second-generation wearable biosensor arrays based on a printed electrode technology involving a water-based graphite ink, for the simultaneous detection of l-lactate and d-glucose. The water-based graphite ink is deposited onto a flexible polyethylene terephthalate sheet, namely stencil-printed graphite (SPG) electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium redox polymer to shuttle the electrons from the redox center of lactate oxidase from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger (GOx). The proposed biosensor array exhibits a limit of detection as low as (9.0 ± 1.0) × 10−6 m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) × 10−6 m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 μA mm−1 for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4 μA mm−1 for GOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested in buffer and artificial sweat and is endowed with an operational/storage stability of ≈80% of the initial signal retained after 20 days. Finally, the proposed array is integrated in a wristband and successfully tested for the continuous monitoring of l-lactate and d-glucose in a healthy volunteer during daily activity. This is foreseen as a real-time wearable device for sport-medicine and healthcare applications.

Original language	English
Journal	Advanced Sensor Research
Volume	3
Issue number	3
DOIs	https://doi.org/10.1002/adsr.202300036
Publication status	Published - 25 Apr 2023
MoE publication type	A1 Journal article-refereed

Funding

The following funding agencies are acknowledged: Academy of Finland projects #316881, #316883 “Spatiotemporal control of Cell Functions,” #332106 “ProSiT—Protein Detection at the Single-Molecule Limit with a Self-powered Organic Transistor for HIV early diagnosis”; Biosensori analitici usa-e getta a base di transistori organici auto-alimentati per la rivelazione di biomarcatori proteomici alla singola molecola per la diagnostica decentrata dell'HIV (6CDD3786); Research for Innovation REFIN—Regione Puglia POR PUGLIA FESR-FSE 2014/2020; PMGB ARS01_01195; NoOne-A binary sensor with single-molecule digit to discriminate biofluids enclosing zero or at least one biomarker, ERC Stg2021, GA:101040383; PRIN project prot.2017RHX2E4 “At the forefront of Analytical ChemisTry: disrUptive detection technoLogies to improve food safety—ACTUaL”; IDF SHARID (ARS01_01270); Åbo Akademi University CoE “Bioelectronic activation of cell functions”; University of Galway College of Science and Engineering Scholarship and CSGI are acknowledged for partial financial support.

Access to Document

10.1002/adsr.202300036Licence: CC BY

Advanced Sensor Research - 2023 - Tricase - Water‐Based Conductive Ink Formulations for Enzyme‐Based Wearable Biosensors (2) (1)Final published version, 1.96 MBLicence: CC BY

https://urn.fi/URN:NBN:fi-fe202502018614

Cite this

@article{66fe71c67dc7404aa72ac16a5c5b7df2,

title = "Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors",

abstract = "Herein, this work reports the first example of second-generation wearable biosensor arrays based on a printed electrode technology involving a water-based graphite ink, for the simultaneous detection of l-lactate and d-glucose. The water-based graphite ink is deposited onto a flexible polyethylene terephthalate sheet, namely stencil-printed graphite (SPG) electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium redox polymer to shuttle the electrons from the redox center of lactate oxidase from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger (GOx). The proposed biosensor array exhibits a limit of detection as low as (9.0 ± 1.0) × 10−6 m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) × 10−6 m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 μA mm−1 for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4 μA mm−1 for GOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested in buffer and artificial sweat and is endowed with an operational/storage stability of ≈80\% of the initial signal retained after 20 days. Finally, the proposed array is integrated in a wristband and successfully tested for the continuous monitoring of l-lactate and d-glucose in a healthy volunteer during daily activity. This is foreseen as a real-time wearable device for sport-medicine and healthcare applications.",

author = "Angelo Tricase and Anna Imbriano and Marlene Valentino and Nicoletta Ditaranto and Eleonora Macchia and \{Di Franco\}, Cinzia and Reshma Kidayaveettil and D{\'o}nal Leech and Matteo Piscitelli and Gaetano Scamarcio and Gaetano Perchiazzi and Luisa Torsi and Paolo Bollella",

year = "2023",

month = apr,

day = "25",

doi = "10.1002/adsr.202300036",

language = "English",

volume = "3",

journal = "Advanced Sensor Research",

issn = "2751-1219",

publisher = "Wiley Open Access",

number = "3",

}

TY - JOUR

T1 - Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors

AU - Tricase, Angelo

AU - Imbriano, Anna

AU - Valentino, Marlene

AU - Ditaranto, Nicoletta

AU - Macchia, Eleonora

AU - Di Franco, Cinzia

AU - Kidayaveettil, Reshma

AU - Leech, Dónal

AU - Piscitelli, Matteo

AU - Scamarcio, Gaetano

AU - Perchiazzi, Gaetano

AU - Torsi , Luisa

AU - Bollella, Paolo

PY - 2023/4/25

Y1 - 2023/4/25

N2 - Herein, this work reports the first example of second-generation wearable biosensor arrays based on a printed electrode technology involving a water-based graphite ink, for the simultaneous detection of l-lactate and d-glucose. The water-based graphite ink is deposited onto a flexible polyethylene terephthalate sheet, namely stencil-printed graphite (SPG) electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium redox polymer to shuttle the electrons from the redox center of lactate oxidase from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger (GOx). The proposed biosensor array exhibits a limit of detection as low as (9.0 ± 1.0) × 10−6 m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) × 10−6 m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 μA mm−1 for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4 μA mm−1 for GOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested in buffer and artificial sweat and is endowed with an operational/storage stability of ≈80% of the initial signal retained after 20 days. Finally, the proposed array is integrated in a wristband and successfully tested for the continuous monitoring of l-lactate and d-glucose in a healthy volunteer during daily activity. This is foreseen as a real-time wearable device for sport-medicine and healthcare applications.

AB - Herein, this work reports the first example of second-generation wearable biosensor arrays based on a printed electrode technology involving a water-based graphite ink, for the simultaneous detection of l-lactate and d-glucose. The water-based graphite ink is deposited onto a flexible polyethylene terephthalate sheet, namely stencil-printed graphite (SPG) electrodes, and further modified with [Os(bpy)2(Cl)(PVI)10] as an osmium redox polymer to shuttle the electrons from the redox center of lactate oxidase from Aerococcus viridans (LOx) and gluocose oxidase from Aspergillus niger (GOx). The proposed biosensor array exhibits a limit of detection as low as (9.0 ± 1.0) × 10−6 m for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and (3.0 ± 0.5) × 10−6 m for GOx/SPG-[Os(bpy)2(Cl)(PVI)10], a sensitivity as high as 1.32 μA mm−1 for LOx/SPG-[Os(bpy)2(Cl)(PVI)10] and 28.4 μA mm−1 for GOx/SPG-[Os(bpy)2(Cl)(PVI)10]. The technology is also selective when tested in buffer and artificial sweat and is endowed with an operational/storage stability of ≈80% of the initial signal retained after 20 days. Finally, the proposed array is integrated in a wristband and successfully tested for the continuous monitoring of l-lactate and d-glucose in a healthy volunteer during daily activity. This is foreseen as a real-time wearable device for sport-medicine and healthcare applications.

U2 - 10.1002/adsr.202300036

DO - 10.1002/adsr.202300036

M3 - Article

SN - 2751-1219

VL - 3

JO - Advanced Sensor Research

JF - Advanced Sensor Research

IS - 3

ER -

Water-Based Conductive Ink Formulations for Enzyme-Based Wearable Biosensors

Abstract

Funding

Access to Document

Fingerprint

Cite this