Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid

null Yatsunami; null Fujiki; null Suganuma; null Yoshizawa; John Eriksson; null Olson; null Goldman

doi:10.1016/0006-291X(91)90662-Q

Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid

Yatsunami, Fujiki, Suganuma, Yoshizawa, John Eriksson, Olson, Goldman

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Abstract

Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.

Original language	Undefined/Unknown
Pages (from-to)	1165–1170
Journal	Biochemical and Biophysical Research Communications
Volume	177
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(91)90662-Q
Publication status	Published - 1991
MoE publication type	A1 Journal article-refereed

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10.1016/0006-291X(91)90662-Q

Cite this

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title = "Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid",

abstract = "Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.",

author = "Yatsunami and Fujiki and Suganuma and Yoshizawa and John Eriksson and Olson and Goldman",

year = "1991",

doi = "10.1016/0006-291X(91)90662-Q",

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volume = "177",

pages = "1165–1170",

journal = "Biochemical and Biophysical Research Communications",

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T1 - Vimentin is hyperphosphorylated in primary human fibroblasts treated with okadaic acid

AU - Yatsunami, null

AU - Fujiki, null

AU - Suganuma, null

AU - Yoshizawa, null

AU - Eriksson, John

AU - Olson, null

AU - Goldman, null

PY - 1991

Y1 - 1991

N2 - Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.

AB - Okadaic acid and dinophysistoxin-1 (35-methylokadaic acid) induced hyperphosphorylation of a 58 kDa protein in primary human fibroblasts, due to inhibition of protein phosphatase 1 and 2A activities. The protein was present in the nuclear and cytosolic fractions. Its pI was 5.3. The hyperphosphorylated protein reacted with monoclonal and polyclonal anti-vimentin antibodies, but not with anti-nucleolin antibody. Phosphorylation of vimentin was stimulated in vitro by dinophysistoxin-1 dose-dependently in the presence of protein phosphatase 2A and protein kinases.

U2 - 10.1016/0006-291X(91)90662-Q

DO - 10.1016/0006-291X(91)90662-Q

M3 - Artikel

SN - 0006-291X

VL - 177

SP - 1165

EP - 1170

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -