UV-induced reaction kinetics of dilinoleoylphosphatidylethanolamine monolayers

Tapani Viitala*, Jouko Peltonen

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

17 Citations (Scopus)

Abstract

The UV-induced reactivity of dilinoleoylphosphatidylethanolamine (DLiPE) Langmuir and Langmuir-Blodgett films has been studied by in situ measurements of the changes n the mean molecular area, UV-vis and Fourier transform infrared spectroscopy, and atomic force microscopy (AFM). Optimum orientation and packing density of the DLiPE molecules in the monolayer were achieved by adding uranyl acetate to the subphase. A first-order reaction kinetic model was successfully fitted to the experimental reaction kinetics data obtained at a surface pressure of 30 mN/m. Topographical studies of LB films by AFM were performed on bilayer structures as a function of subphase composition and UV irradiation time. The orientational effect of the uranyl ions on the monolayer molecules was observed as an enhanced homogeneity of the freshly prepared monomeric LB films. However, the long-term stability of these films proved to be bad; clear reorganization and loss of a true monolayer structure were evidenced by the AFM images. This instability was inhibited for the UV- irradiated films, indicating that the UV irradiation gave rise to a cross- linked structure.

Original languageEnglish
Pages (from-to)2803-2813
Number of pages11
JournalBiophysical Journal
Volume76
Issue number5
DOIs
Publication statusPublished - 1999
MoE publication typeA1 Journal article-refereed

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