Transcriptional profiles and structural models of the Synechocystis sp. PCC 6803 Deg proteases

Tove Jansén, Heidi Kidron, Hanna Taipaleenmäki, Tiina Salminen, Pirkko Mäenpää

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

The Synechocystis sp. PCC 6803 genome harbours a deg gene family consisting of three members, degP (htrA, slr1204), degQ (hhoA, sll1679) and degS (hhoB, sll1427). We studied the environmental regulation of the Synechocystis sp. PCC 6803 deg genes at the level of transcription and protein structures of the gene products to evaluate their hypothetical role in D1 protein turnover. Northern blotting showed that transcription of the deg genes is differentially regulated, supporting a view of distinct roles of Degs in cellular processes. The oligomerization state as well as the three dimensional structures of the Synechocystis sp. PCC 6803 Deg proteases were predicted based on an amino acid sequence alignment and comparison of the Deg crystal structures from human, Escherichia coli and Thermotoga maritima. The structures of the Synechocystis sp. PCC 6803 Degs resemble more the Thermotoga maritima Deg enzyme structure than the Escherichia coli one. Moreover, the structures of the LA-loops hint towards a homotrimeric form of the Synechocystis sp. PCC 6803 Deg proteases.

Original languageEnglish
Pages (from-to)57-63
Number of pages7
JournalPhotosynthesis Research
Volume84
Issue number1-3
DOIs
Publication statusPublished - Jun 2005
MoE publication typeA1 Journal article-refereed

Keywords

  • Amino Acid Sequence
  • Gene Expression Profiling
  • Gene Expression Regulation, Bacterial
  • Models, Molecular
  • Molecular Sequence Data
  • Multigene Family
  • Peptide Hydrolases/chemistry
  • Protein Conformation
  • Sequence Homology, Amino Acid
  • Synechocystis/enzymology
  • Transcription, Genetic

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