Topochemistry of Environmentally Friendly Pretreatments To Enhance Enzymatic Hydrolysis of Sugar Cane Bagasse to Fermentable Sugar

Hongyan Mou, Elina Heikkilä, Pedro Fardim

    Research output: Contribution to journalArticleScientificpeer-review

    26 Citations (Scopus)


    In this work, dilute alkaline and alkaline peroxide pretreatments were conducted in comparison with hydrotropic pretreatment to improve the delignification of bagasse prior to enzymatic hydrolysis. The surface chemical composition of bagasse after pretreatments was investigated by X-ray photoelectron spectroscopy (XPS) and time-of-flight secondary ion mass spectrometry (ToF-SIMS). The surface distribution of lignin and extractives on the bagasse fiber was significantly changed by dilute alkaline, alkaline peroxide, and hydrotropic pretreatments. Hydrotropic pretreatment typically showed, other than the decrease of surface coverage by lignin and extractives, dramatic removal of xylan, thereby leading to more cellulose exposed on the fiber surface after pretreatment. Fiber morphology after pretreatments was more favorable for enzyme hydrolysis as well. However, the hydrotropic treatment had clear advantages because the enzymatic hydrolysis yields of glucan and xylan of pretreated bagasse were 83.9 and 14.3%, respectively.
    Original languageUndefined/Unknown
    Pages (from-to)3619–3625
    Number of pages7
    JournalJournal of Agricultural and Food Chemistry
    Issue number16
    Publication statusPublished - 2014
    MoE publication typeA1 Journal article-refereed


    • enzymatic hydrolysis
    • hydrotropic pretreatment
    • sugar cane bagasse
    • surface chemistry
    • ToF-SIMS
    • XPS

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