The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency

MR Emani; E Närvä; A Stubb; D Chakroborty; M Viitala; A Rokka; N Rahkonen; R Moulder; Konstantin Denessiouk; R Trokovic; R Lund; LL Elo; R. Lahesmaa

The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency

MR Emani, E Närvä, A Stubb, D Chakroborty, M Viitala, A Rokka, N Rahkonen, R Moulder, Konstantin Denessiouk, R Trokovic, R Lund, LL Elo, R. Lahesmaa

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Abstract

The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.

Original language	Undefined/Unknown
Pages (from-to)	519–528
Journal	Stem Cell Reports
Volume	4
Issue number	3
Publication status	Published - 2015
MoE publication type	A1 Journal article-refereed

Keywords

Protein Interactome

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Cite this

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title = "The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency",

abstract = "The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.",

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TY - JOUR

T1 - The L1TD1 protein interactome reveals the importance of post-transcriptional regulation in human pluripotency

AU - Emani, MR

AU - Närvä, E

AU - Stubb, A

AU - Chakroborty, D

AU - Viitala, M

AU - Rokka, A

AU - Rahkonen, N

AU - Moulder, R

AU - Denessiouk, Konstantin

AU - Trokovic, R

AU - Lund, R

AU - Elo, LL

AU - Lahesmaa, R.

PY - 2015

Y1 - 2015

N2 - The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.

AB - The RNA-binding protein L1TD1 is one of the most specific and abundant proteins in pluripotent stem cells and is essential for the maintenance of pluripotency in human cells. Here, we identify the protein interaction network of L1TD1 in human embryonic stem cells (hESCs) and provide insights into the interactome network constructed in human pluripotent cells. Our data reveal that L1TD1 has an important role in RNA splicing, translation, protein traffic, and degradation. L1TD1 interacts with multiple stem-cell-specific proteins, many of which are still uncharacterized in the context of development. Further, we show that L1TD1 is a part of the pluripotency interactome network of OCT4, SOX2, and NANOG, bridging nuclear and cytoplasmic regulation and highlighting the importance of RNA biology in pluripotency.

KW - Protein Interactome

M3 - Artikel

SN - 2213-6711

VL - 4

SP - 519

EP - 528

JO - Stem Cell Reports

JF - Stem Cell Reports

IS - 3

ER -