Synthesis and organotropism of 3H-dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin

Lisa Spoof, Svetlana Klimova, Andrey Mikhailov, John E. Eriksson, Jussi Meriluoto*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

12 Citations (Scopus)

Abstract

Tritium-labelled dihydro derivatives of the cyanobacterial peptide hepatotoxin nodularin were prepared by reduction with sodium boro[3H]hydride. The optimised reaction gave two dihydronodularin stereoisomers which were purified by high-performance liquid chromatography with a mobile phase of methanol-0.7% sodium sulfate (6:4) and a C18 stationary phase. The specific activities of the stereoisomers were 1780-1807 dis min-1 ng-1. The radiolabelled dihydronodularins were tested for stability and used for toxicokinetic studies in mice. Liver was the main site of toxin accumulation.

Original languageEnglish
Pages (from-to)153-162
Number of pages10
JournalToxicon
Volume41
Issue number2
DOIs
Publication statusPublished - 1 Feb 2003
MoE publication typeA1 Journal article-refereed

Keywords

  • Cyanobacteria
  • Nodularin
  • Organotropism
  • Radiolabelling
  • Toxin

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