Study of the sterile conk of Inonotus obliquus using 13C CPMAS NMR and FTIR spectroscopies coupled with multivariate analysis

Gabriele Beltrame*, Ida Mattsson, Pia Damlin, Zenghua Han, Carita Kvarnström, Reko Leino, Baoru Yang

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

1 Citation (Scopus)

Abstract

The sterile conk caused by the infection of the basidiomycete Inonotus obliquus (Chaga) is an important source of bioactive compounds. However, its structure and biochemistry are only generally understood. Solid state 13C NMR and FTIR spectroscopy have been utilized for the first time to investigate the sterile conk with non-invasive methods. The application of multivariate data analysis techniques and spectral distance algorithm to the obtained datasets showed clear distinction between the outer and inner layers of the sterile conk. Moreover, the sterile conk bark, compared to the inner layers, was spectroscopically more similar to wood tissues. The fungal tissue was proven to be concentrated below the bark. The similarity of the sterile conk inner layers to both decayed wood and hyphae of I. obliquus was shown by the multivariate data analysis of both spectra datasets. The spectroscopic data indicated lack of lignin degradation in the heart rot, except for demethoxylation, and a slight preference for hemicellulose degradation. Therefore, the results obtained suggest that the classification of I. obliquus as preferential lignin degrader (white-rot fungus) should be revised and clarified by further studies.

Original languageEnglish
Article number133226
JournalJournal of Molecular Structure
Volume1264
DOIs
Publication statusPublished - 15 Sep 2022
MoE publication typeA1 Journal article-refereed

Keywords

  • C CPMAS
  • FTIR
  • Inonotus obliquus
  • Spectral distance
  • Sterile conk

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