Structure of anionic phospholipid coatings on silica by dissipative quartz crystal microbalance

Tapani Viitala*, Jari T. Hautala, Jorma Vuorinen, Susanne K. Wiedmer

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

69 Citations (Scopus)


The adsorption of anionic phospholipids on silica was investigated by the dissipative quartz crystal microbalance (QCM) technique. Liposomes composed of 1 mM 80:20 mol % of 1 -palmitoyl-2-oley1-sn-glycero-3-phosphatidylcholine (POPC)/phosphatidic acid, POPC/phosphatidylglycerol, or POPC/phosphatidylserine in N-(2-hydroxyethyl)piperazineN′-(2-ethanesulfonic acid) buffer at pH 7.4 (with or without 3 mM of CaCl2) were examined. We have previously demonstrated that similar phospholipid coatings can be used in capillary electrochromatography as a stationary phase for the separation of analytes. In this work, we focus on the formation of the coatings and on the type of lipid structure formed on silica. The QCM investigation comprised qualitative results based on changes in frequency and resistance, and quantitative modeling of the obtained results. The latter was performed using the dissipative QCM, which measures the quartz crystal impedance, combined with equivalent circuit analysis. A previously developed coating and cleaning procedure for phospholipid-coated fused silica capillaries was adopted in this study, and the same silica-coated crystal was used throughout the QCM study. We will demonstrate in this work that the type of lipid structure formed on silica, that is, a rather rigid supported lipid bilayer or a viscoelastic supported vesicle layer (SVL), is highly dependent on the lipid and solvent composition. We also show for the first time that the modeling of the dissipative QCM data can be used to extract a more quantitative picture of an adsorbed SVL, because, so far, published studies have merely used the QCM data in a qualitative sense.

Original languageEnglish
Pages (from-to)609-618
Number of pages10
Issue number2
Publication statusPublished - 16 Jan 2007
Externally publishedYes
MoE publication typeA1 Journal article-refereed


Dive into the research topics of 'Structure of anionic phospholipid coatings on silica by dissipative quartz crystal microbalance'. Together they form a unique fingerprint.

Cite this