Stressor-dependent regulation of the heat shock response in zebrafish, Danio rerio

Susanna Airaksinen, Christina M I Råbergh, Anna Lahti, Annukka Kaatrasalo, Lea Sistonen, Mikko Nikinmaa

Research output: Contribution to journalArticleScientificpeer-review

33 Citations (Scopus)


Heat shock transcription factors (HSFs) regulate expression of heat shock proteins (Hsps). We have previously shown that in zebrafish a unique isoform, zHSF1b, disappears concomitant with heat shock-induced Hsp70 expression. To characterize the role of zHSF1a and zHSF1b isoforms in the regulation of the stress response in vivo, we have carried out cadmium (10-100 microM) and copper (10-30 microM) exposures in order to specify whether the disappearance of HSF1b is specific for heat stress. After 4-h metal exposures we analyzed the expression of hsp70, zHSF1a, zHSF1b and metallothionein (MT) by reverse transcriptase polymerase chain reaction in zebrafish liver, gonads and gills. Although cadmium is a known inducer of Hsps, it did not affect hsp70 expression significantly in the studied tissues. Induction of hsp70 was observed upon copper exposure in liver and gonads, but not in gills. Neither metal affected the zHSF1a/b ratio. Both cadmium and copper exposure caused upregulation of MT, regulator of metal homeostasis and detoxification, confirming that the tissues were subjected to metal loads. Thus, hsp70 appears to be more weakly induced upon metal exposure than in response to heat shock and HSF1 isoforms may participate in stressor-specific regulation of hsp70.

Original languageEnglish
Pages (from-to)839-46
Number of pages8
JournalComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiology
Issue number4
Publication statusPublished - Apr 2003
MoE publication typeA1 Journal article-refereed


  • Animals
  • DNA-Binding Proteins/genetics
  • Gene Expression Regulation
  • HSP70 Heat-Shock Proteins/genetics
  • Heat Shock Transcription Factors
  • Heat-Shock Response
  • Reverse Transcriptase Polymerase Chain Reaction
  • Transcription Factors
  • Zebrafish/physiology


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