Spheroid culture of LuCaP 136 patient-derived xenograft enables versatile preclinical models of prostate cancer

MP Valta, HJ Zhao, M Saar, J Tuomela, R Nolley, J Linxweiler, Jouko Sandholm, J Lehtimaki, P Harkonen, I Coleman, PS Nelson, E Corey, DM Peehl

Research output: Contribution to journalArticleScientificpeer-review

10 Citations (Scopus)

Abstract

LuCaP serially transplantable patient-derived xenografts (PDXs) are valuable preclinical models of locally advanced or metastatic prostate cancer. Using spheroid culture methodology, we recently established cell lines from several LuCaP PDXs. Here, we characterized in depth the features of xenografts derived from LuCaP 136 spheroid cultures and found faithful retention of the phenotype of the original PDX. In vitro culture enabled luciferase transfection into LuCaP 136 spheroids, facilitating in vivo imaging. We showed that LuCaP 136 spheroids formed intratibial, orthotopic, and subcutaneous tumors when re-introduced into mice. Intratibial tumors responded to castration and were highly osteosclerotic. LuCaP 136 is a realistic in vitro-in vivo preclinical model of a subtype of bone metastatic prostate cancer.
Original languageUndefined/Unknown
Pages (from-to)325–337
Number of pages13
JournalClinical and Experimental Metastasis
Volume33
Issue number4
DOIs
Publication statusPublished - 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • Androgen-dependence
  • Bone metastasis
  • Osteosclerosis
  • Prostate cancer

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