Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Cecilia Scandurra; Kim Björkström; Lucia Sarcina; Anna Imbriano; Cinzia Di Franco; Ronald Österbacka; Paolo Bolella; Gaetano Scamarcio; Luisa Torsi; Eleonora Macchia

doi:10.1002/admt.202201910

Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Cecilia Scandurra, Kim Björkström, Lucia Sarcina, Anna Imbriano, Cinzia Di Franco, Ronald Österbacka, Paolo Bolella, Gaetano Scamarcio, Luisa Torsi^*, Eleonora Macchia^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

Early diagnosis and efficient treatments of oncological, neurological, inflammatory, and infectious diseases rely more and more on ultrasensitive detection of protein markers; the ultimate limit is a reliable immunoassay capable of single-protein detection. Among protein biomarkers, cytokines play a key role in clinical diagnosis as they are involved in developing many complex diseases and disorders, such as chronic inflammatory diseases including metabolic syndrome, neurodegenerative diseases, and cardiovascular diseases, along with autoimmune diseases and cancer. Herein, the improvement of a Single Molecule with Transistor (SiMoT) is reported based on an electrolyte-gated organic field-effect transistor applied for the detection of cytokine IL-6 in blood serum, reaching a limit-of-detection (LOD) of 1 ± 1 protein in a sample of 0.1 mL. The analytical performance levels are benchmarked against the Simoa Planar Array SP-X technology, a benchtop chemiluminescent array. After comprehensive optimization, Simoa SP-X by using a multivariate experimental design approach exhibits a LOD 10³ higher than SiMoT. The proposed SiMoT electronic assay is label-free, fast (30 min), and selective, paving the way for an ultra-sensitive point-of-care immunoassay platform enabling pre-symptomatic disease diagnosis.

Original language	English
Article number	2201910
Journal	Advanced Materials Technologies
Volume	8
Issue number	11
DOIs	https://doi.org/10.1002/admt.202201910
Publication status	Published - 9 Jun 2023
MoE publication type	A1 Journal article-refereed

Keywords

cytokine IL-6 detection
electrolyte-gated organic field-effect transistors
sandwich immunoassays
Simoa SP-X technology
single-molecule assay with a large transistor (SiMoT)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1002/admt.202201910Licence: CC BY

Adv Materials Technologies - 2023 - Scandurra - Single Molecule with a Large Transistor SiMoT cytokine IL‐6 DetectionFinal published version, 903 KBLicence: CC BY

https://urn.fi/URN:NBN:fi-fe2023053049465

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Scandurra, C., Björkström, K., Sarcina, L., Imbriano, A., Di Franco, C., Österbacka, R., Bolella, P., Scamarcio, G., Torsi, L., & Macchia, E. (2023). Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array. Advanced Materials Technologies, 8(11), Article 2201910. https://doi.org/10.1002/admt.202201910

@article{c026299ff252454aaeb4e1cef449078b,

title = "Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array",

abstract = "Early diagnosis and efficient treatments of oncological, neurological, inflammatory, and infectious diseases rely more and more on ultrasensitive detection of protein markers; the ultimate limit is a reliable immunoassay capable of single-protein detection. Among protein biomarkers, cytokines play a key role in clinical diagnosis as they are involved in developing many complex diseases and disorders, such as chronic inflammatory diseases including metabolic syndrome, neurodegenerative diseases, and cardiovascular diseases, along with autoimmune diseases and cancer. Herein, the improvement of a Single Molecule with Transistor (SiMoT) is reported based on an electrolyte-gated organic field-effect transistor applied for the detection of cytokine IL-6 in blood serum, reaching a limit-of-detection (LOD) of 1 ± 1 protein in a sample of 0.1 mL. The analytical performance levels are benchmarked against the Simoa Planar Array SP-X technology, a benchtop chemiluminescent array. After comprehensive optimization, Simoa SP-X by using a multivariate experimental design approach exhibits a LOD 103 higher than SiMoT. The proposed SiMoT electronic assay is label-free, fast (30 min), and selective, paving the way for an ultra-sensitive point-of-care immunoassay platform enabling pre-symptomatic disease diagnosis.",

keywords = "cytokine IL-6 detection, electrolyte-gated organic field-effect transistors, sandwich immunoassays, Simoa SP-X technology, single-molecule assay with a large transistor (SiMoT)",

author = "Cecilia Scandurra and Kim Bj{\"o}rkstr{\"o}m and Lucia Sarcina and Anna Imbriano and {Di Franco}, Cinzia and Ronald {\"O}sterbacka and Paolo Bolella and Gaetano Scamarcio and Luisa Torsi and Eleonora Macchia",

note = "Funding Information: H2020 – Electronic Smart Systems – SiMBiT: Single‐molecule bio‐electronic smart system array for clinical testing (Grant agreement ID: 824946), NoOne‐A binary sensor with a single‐molecule digit to discriminate biofluids enclosing zero or at least one biomarker, ERC Stg2021, GA:101040383, Biosensori analitici usa‐e getta a base di transistori organici auto‐alimentati per la rivelazione di biomarcatori proteomici alla singola molecola per la diagnostica decentrata dell'HIV (6CDD3786); Research for Innovation REFIN—Regione Puglia POR PUGLIA FESR‐FSE 2014/2020, PMGB—Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l'oncologia di precisione—ARS01_01195‐PON “RICERCA E INNOVAZIONE” 2014–2020, {\AA}bo Akademi University CoE “Bioelectronic activation of cell functions”, Svenska Tekniska Vetenskapsakademien i Finland, and CSGI are acknowledged for partial financial support. Publisher Copyright: {\textcopyright} 2023 The Authors. Advanced Materials Technologies published by Wiley-VCH GmbH.",

year = "2023",

month = jun,

day = "9",

doi = "10.1002/admt.202201910",

language = "English",

volume = "8",

journal = "Advanced Materials Technologies",

issn = "2365-709X",

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Scandurra, C, Björkström, K, Sarcina, L, Imbriano, A, Di Franco, C, Österbacka, R, Bolella, P, Scamarcio, G, Torsi, L & Macchia, E 2023, 'Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array', Advanced Materials Technologies, vol. 8, no. 11, 2201910. https://doi.org/10.1002/admt.202201910

TY - JOUR

T1 - Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

AU - Scandurra, Cecilia

AU - Björkström, Kim

AU - Sarcina, Lucia

AU - Imbriano, Anna

AU - Di Franco, Cinzia

AU - Österbacka, Ronald

AU - Bolella, Paolo

AU - Scamarcio, Gaetano

AU - Torsi, Luisa

AU - Macchia, Eleonora

N1 - Funding Information: H2020 – Electronic Smart Systems – SiMBiT: Single‐molecule bio‐electronic smart system array for clinical testing (Grant agreement ID: 824946), NoOne‐A binary sensor with a single‐molecule digit to discriminate biofluids enclosing zero or at least one biomarker, ERC Stg2021, GA:101040383, Biosensori analitici usa‐e getta a base di transistori organici auto‐alimentati per la rivelazione di biomarcatori proteomici alla singola molecola per la diagnostica decentrata dell'HIV (6CDD3786); Research for Innovation REFIN—Regione Puglia POR PUGLIA FESR‐FSE 2014/2020, PMGB—Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l'oncologia di precisione—ARS01_01195‐PON “RICERCA E INNOVAZIONE” 2014–2020, Åbo Akademi University CoE “Bioelectronic activation of cell functions”, Svenska Tekniska Vetenskapsakademien i Finland, and CSGI are acknowledged for partial financial support. Publisher Copyright: © 2023 The Authors. Advanced Materials Technologies published by Wiley-VCH GmbH.

PY - 2023/6/9

Y1 - 2023/6/9

N2 - Early diagnosis and efficient treatments of oncological, neurological, inflammatory, and infectious diseases rely more and more on ultrasensitive detection of protein markers; the ultimate limit is a reliable immunoassay capable of single-protein detection. Among protein biomarkers, cytokines play a key role in clinical diagnosis as they are involved in developing many complex diseases and disorders, such as chronic inflammatory diseases including metabolic syndrome, neurodegenerative diseases, and cardiovascular diseases, along with autoimmune diseases and cancer. Herein, the improvement of a Single Molecule with Transistor (SiMoT) is reported based on an electrolyte-gated organic field-effect transistor applied for the detection of cytokine IL-6 in blood serum, reaching a limit-of-detection (LOD) of 1 ± 1 protein in a sample of 0.1 mL. The analytical performance levels are benchmarked against the Simoa Planar Array SP-X technology, a benchtop chemiluminescent array. After comprehensive optimization, Simoa SP-X by using a multivariate experimental design approach exhibits a LOD 103 higher than SiMoT. The proposed SiMoT electronic assay is label-free, fast (30 min), and selective, paving the way for an ultra-sensitive point-of-care immunoassay platform enabling pre-symptomatic disease diagnosis.

AB - Early diagnosis and efficient treatments of oncological, neurological, inflammatory, and infectious diseases rely more and more on ultrasensitive detection of protein markers; the ultimate limit is a reliable immunoassay capable of single-protein detection. Among protein biomarkers, cytokines play a key role in clinical diagnosis as they are involved in developing many complex diseases and disorders, such as chronic inflammatory diseases including metabolic syndrome, neurodegenerative diseases, and cardiovascular diseases, along with autoimmune diseases and cancer. Herein, the improvement of a Single Molecule with Transistor (SiMoT) is reported based on an electrolyte-gated organic field-effect transistor applied for the detection of cytokine IL-6 in blood serum, reaching a limit-of-detection (LOD) of 1 ± 1 protein in a sample of 0.1 mL. The analytical performance levels are benchmarked against the Simoa Planar Array SP-X technology, a benchtop chemiluminescent array. After comprehensive optimization, Simoa SP-X by using a multivariate experimental design approach exhibits a LOD 103 higher than SiMoT. The proposed SiMoT electronic assay is label-free, fast (30 min), and selective, paving the way for an ultra-sensitive point-of-care immunoassay platform enabling pre-symptomatic disease diagnosis.

KW - cytokine IL-6 detection

KW - electrolyte-gated organic field-effect transistors

KW - sandwich immunoassays

KW - Simoa SP-X technology

KW - single-molecule assay with a large transistor (SiMoT)

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U2 - 10.1002/admt.202201910

DO - 10.1002/admt.202201910

M3 - Article

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SN - 2365-709X

VL - 8

JO - Advanced Materials Technologies

JF - Advanced Materials Technologies

IS - 11

M1 - 2201910

ER -

Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Åbo Akademi Functional Printing Center

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions

SIMBIT: Single molecule bio-electronic smart system array for clinical testing

SPACE: Spatiotemporal Control of Cell Functions

Cite this

Single Molecule with a Large Transistor – SiMoT cytokine IL-6 Detection Benchmarked against a Chemiluminescent Ultrasensitive Immunoassay Array

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Equipment

Åbo Akademi Functional Printing Center

Projects

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions

SIMBIT: Single molecule bio-electronic smart system array for clinical testing

SPACE: Spatiotemporal Control of Cell Functions

Cite this