Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum

Eleonora Macchia; Lucia Sarcina; Caroline Driescher; Zahra Gounani; Amit Tewari; Ronald Osterbacka; Gerardo Palazzo; Angelo Tricase; Zsolt Miklos Kovacs Vajna; Fabrizio Viola; Francesco Modena; Mario Caironi; Fabrizio Torricelli; Irene Esposito; Luisa Torsi

doi:10.1002/aelm.202100304

Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum

Eleonora Macchia, Lucia Sarcina, Caroline Driescher, Zahra Gounani, Amit Tewari, Ronald Osterbacka, Gerardo Palazzo, Angelo Tricase, Zsolt Miklos Kovacs Vajna, Fabrizio Viola, Francesco Modena, Mario Caironi, Fabrizio Torricelli, Irene Esposito, Luisa Torsi^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

25 Citations (Scopus)

Abstract

The timely diagnosis of cystic pancreatic cancer precursors is of utmost importance to improve patients’ low survival rate. Fine-needle aspiration cytology is endowed with low diagnostic sensitivity, while more effective is the assay of markers, such as a mutated KRAS, in the cyst fluids. Next-generation sequencing, detecting down to a single copy of a genomic marker, enables early diagnosis but the diagnostic sensitivity of high-grade cysts, likely to become malignant, is low. Assaying both mutated KRAS and MUC1 protein markers can improve diagnostic accuracy. Their detection in blood would also be minimally invasive. Here, the mucinous lesions markers, KRAS and MUC1, are both successfully assayed in blood serum at the physical limit with the label-free “Single-Molecule assay with a large Transistor—SiMoT.” This is a compelling proof of principle that the SiMoT platform holds high potential to enable a timely, minimally invasive, and accurate diagnosis of pancreatic cancer precursor cysts.

Original language	English
Article number	2100304
Number of pages	11
Journal	Advanced Electronic Materials
Volume	7
Issue number	9
DOIs	https://doi.org/10.1002/aelm.202100304
Publication status	Published - Sept 2021
MoE publication type	A1 Journal article-refereed

Keywords

electrolyte-gated field-effect-transistors
organic bioelectronic sensors
pancreatic cancer
pancreatic cysts
single-molecule assay

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1002/aelm.202100304

Cite this

Macchia, E., Sarcina, L., Driescher, C., Gounani, Z., Tewari, A., Osterbacka, R., Palazzo, G., Tricase, A., Kovacs Vajna, Z. M., Viola, F., Modena, F., Caironi, M., Torricelli, F., Esposito, I., & Torsi, L. (2021). Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum. Advanced Electronic Materials, 7(9), Article 2100304. https://doi.org/10.1002/aelm.202100304

@article{fff04f4897d34ca18719b95877c248ca,

title = "Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts{\textquoteright} in Whole Blood Serum",

abstract = "The timely diagnosis of cystic pancreatic cancer precursors is of utmost importance to improve patients{\textquoteright} low survival rate. Fine-needle aspiration cytology is endowed with low diagnostic sensitivity, while more effective is the assay of markers, such as a mutated KRAS, in the cyst fluids. Next-generation sequencing, detecting down to a single copy of a genomic marker, enables early diagnosis but the diagnostic sensitivity of high-grade cysts, likely to become malignant, is low. Assaying both mutated KRAS and MUC1 protein markers can improve diagnostic accuracy. Their detection in blood would also be minimally invasive. Here, the mucinous lesions markers, KRAS and MUC1, are both successfully assayed in blood serum at the physical limit with the label-free “Single-Molecule assay with a large Transistor—SiMoT.” This is a compelling proof of principle that the SiMoT platform holds high potential to enable a timely, minimally invasive, and accurate diagnosis of pancreatic cancer precursor cysts.",

keywords = "electrolyte-gated field-effect-transistors, organic bioelectronic sensors, pancreatic cancer, pancreatic cysts, single-molecule assay",

author = "Eleonora Macchia and Lucia Sarcina and Caroline Driescher and Zahra Gounani and Amit Tewari and Ronald Osterbacka and Gerardo Palazzo and Angelo Tricase and {Kovacs Vajna}, {Zsolt Miklos} and Fabrizio Viola and Francesco Modena and Mario Caironi and Fabrizio Torricelli and Irene Esposito and Luisa Torsi",

note = "Funding Information: Prof. Eugenio Cantatore, Dr. Piero Larizza, Dr. Guillaume Fichet, and Prof. Gaetano Scamarcio are acknowledged for useful discussions. SiMBiT—Single molecule bioelectronic smart system array for clinical testing (Grant Agreement ID: 824946), PMGB ‐Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l'oncologia di precisione—ARS01_01195—PON “RICERCA E INNOVAZIONE” 2014–2020, Academy of Finland projects #316881, #316883 “Spatiotemporal control of Cell Functions,”, #332106 “ProSiT—Protein Detection at the Single‐Molecule Limit with a Self‐powered Organic Transistor for HIV early diagnosis,” {\AA}bo Akademi University CoE “Bioelectronic activation of cell functions” and CSGI are acknowledged for partial financial support. Publisher Copyright: {\textcopyright} 2021 The Authors. Advanced Electronic Materials published by Wiley-VCH GmbH",

year = "2021",

month = sep,

doi = "10.1002/aelm.202100304",

language = "English",

volume = "7",

journal = "Advanced Electronic Materials",

issn = "2199-160X",

publisher = "Wiley",

number = "9",

}

Macchia, E, Sarcina, L, Driescher, C, Gounani, Z, Tewari, A, Osterbacka, R, Palazzo, G, Tricase, A, Kovacs Vajna, ZM, Viola, F, Modena, F, Caironi, M, Torricelli, F, Esposito, I & Torsi, L 2021, 'Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum', Advanced Electronic Materials, vol. 7, no. 9, 2100304. https://doi.org/10.1002/aelm.202100304

TY - JOUR

T1 - Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum

AU - Macchia, Eleonora

AU - Sarcina, Lucia

AU - Driescher, Caroline

AU - Gounani, Zahra

AU - Tewari, Amit

AU - Osterbacka, Ronald

AU - Palazzo, Gerardo

AU - Tricase, Angelo

AU - Kovacs Vajna, Zsolt Miklos

AU - Viola, Fabrizio

AU - Modena, Francesco

AU - Caironi, Mario

AU - Torricelli, Fabrizio

AU - Esposito, Irene

AU - Torsi, Luisa

N1 - Funding Information: Prof. Eugenio Cantatore, Dr. Piero Larizza, Dr. Guillaume Fichet, and Prof. Gaetano Scamarcio are acknowledged for useful discussions. SiMBiT—Single molecule bioelectronic smart system array for clinical testing (Grant Agreement ID: 824946), PMGB ‐Sviluppo di piattaforme meccatroniche, genomiche e bioinformatiche per l'oncologia di precisione—ARS01_01195—PON “RICERCA E INNOVAZIONE” 2014–2020, Academy of Finland projects #316881, #316883 “Spatiotemporal control of Cell Functions,”, #332106 “ProSiT—Protein Detection at the Single‐Molecule Limit with a Self‐powered Organic Transistor for HIV early diagnosis,” Åbo Akademi University CoE “Bioelectronic activation of cell functions” and CSGI are acknowledged for partial financial support. Publisher Copyright: © 2021 The Authors. Advanced Electronic Materials published by Wiley-VCH GmbH

PY - 2021/9

Y1 - 2021/9

N2 - The timely diagnosis of cystic pancreatic cancer precursors is of utmost importance to improve patients’ low survival rate. Fine-needle aspiration cytology is endowed with low diagnostic sensitivity, while more effective is the assay of markers, such as a mutated KRAS, in the cyst fluids. Next-generation sequencing, detecting down to a single copy of a genomic marker, enables early diagnosis but the diagnostic sensitivity of high-grade cysts, likely to become malignant, is low. Assaying both mutated KRAS and MUC1 protein markers can improve diagnostic accuracy. Their detection in blood would also be minimally invasive. Here, the mucinous lesions markers, KRAS and MUC1, are both successfully assayed in blood serum at the physical limit with the label-free “Single-Molecule assay with a large Transistor—SiMoT.” This is a compelling proof of principle that the SiMoT platform holds high potential to enable a timely, minimally invasive, and accurate diagnosis of pancreatic cancer precursor cysts.

AB - The timely diagnosis of cystic pancreatic cancer precursors is of utmost importance to improve patients’ low survival rate. Fine-needle aspiration cytology is endowed with low diagnostic sensitivity, while more effective is the assay of markers, such as a mutated KRAS, in the cyst fluids. Next-generation sequencing, detecting down to a single copy of a genomic marker, enables early diagnosis but the diagnostic sensitivity of high-grade cysts, likely to become malignant, is low. Assaying both mutated KRAS and MUC1 protein markers can improve diagnostic accuracy. Their detection in blood would also be minimally invasive. Here, the mucinous lesions markers, KRAS and MUC1, are both successfully assayed in blood serum at the physical limit with the label-free “Single-Molecule assay with a large Transistor—SiMoT.” This is a compelling proof of principle that the SiMoT platform holds high potential to enable a timely, minimally invasive, and accurate diagnosis of pancreatic cancer precursor cysts.

KW - electrolyte-gated field-effect-transistors

KW - organic bioelectronic sensors

KW - pancreatic cancer

KW - pancreatic cysts

KW - single-molecule assay

UR - http://www.scopus.com/inward/record.url?scp=85108191205&partnerID=8YFLogxK

U2 - 10.1002/aelm.202100304

DO - 10.1002/aelm.202100304

M3 - Article

AN - SCOPUS:85108191205

SN - 2199-160X

VL - 7

JO - Advanced Electronic Materials

JF - Advanced Electronic Materials

IS - 9

M1 - 2100304

ER -

Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

ProSiT: Protein Detection at the Single Molecule Limit with a Self-powered Organic Transistor for HIV early diagnosis

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions

SIMBIT: Single molecule bio-electronic smart system array for clinical testing

Cite this

Single-Molecule Bioelectronic Label-Free Assay of both Protein and Genomic Markers of Pancreatic Mucinous Cysts’ in Whole Blood Serum

Abstract

Keywords

UN SDGs

Access to Document

Other files and links

Fingerprint

Projects

ProSiT: Protein Detection at the Single Molecule Limit with a Self-powered Organic Transistor for HIV early diagnosis

BACE: Center of Excellence in Bioelectronic Activation of Cell Functions

SIMBIT: Single molecule bio-electronic smart system array for clinical testing

Cite this