Scots pine (Pinus sylvestris) bark composition and degradation by fungi: Potential substrate for bioremediation

Lara Valentín; Beata Kluczek-Turpeinen; Stefan Willför; Jarl Hemming; Annele Hatakka; Kari Steffen; Marja Tuomela

doi:10.1016/j.biortech.2009.11.052

Scots pine (Pinus sylvestris) bark composition and degradation by fungi: Potential substrate for bioremediation

Lara Valentín^*, Beata Kluczek-Turpeinen, Stefan Willför, Jarl Hemming, Annele Hatakka, Kari Steffen, Marja Tuomela

^*Corresponding author for this work

Åbo Akademi University

Research output: Contribution to journal › Article › Scientific › peer-review

86 Citations (Scopus)

Abstract

The composition of Scots pine bark, its degradation, and the production of hydrolytic and ligninolytic enzymes were evaluated during 90 days of incubation with Phanerochaete velutina and Stropharia rugosoannulata. The aim was to evaluate if pine bark can be a suitable fungal substrate for bioremediation applications. The original pine bark contained 45% lignin, 25% cellulose, and 15% hemicellulose. Resin acids were the most predominant lipophilic extractives, followed by sitosterol and unsaturated fatty acids, such as linoleic and oleic acids. Both fungi degraded all main components of bark, specially cellulose (79% loss by P. velutina). During cultivation on pine bark, fungi also degraded sitosterol, produced malic acid, and oxidated unsaturated fatty acids. The most predominant enzymes produced by both fungi were cellulase and manganese peroxidase. The results indicate that Scots pine bark supports enzyme production and provides nutrients to fungi, thus pine bark may be suitable fungal substrate for bioremediation.

Original language	English
Pages (from-to)	2203-2209
Number of pages	7
Journal	Bioresource Technology
Volume	101
Issue number	7
DOIs	https://doi.org/10.1016/j.biortech.2009.11.052
Publication status	Published - Apr 2010
MoE publication type	A1 Journal article-refereed

Funding

Author Lara Valentín thanks the Finnish Graduate School of Environmental Science and Technology (EnSTe) for supporting her PhD studies. This study was funded by Niska ja Nyyssönen Oy and by the Finnish Funding Agency for Technology and Innovation (TEKES) through SYMBIO program.

Keywords

Extractives
Hydrolytic enzymes
Manganese peroxidase
Phanerochaete velutina
Stropharia rugosoannulata

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title = "Scots pine (Pinus sylvestris) bark composition and degradation by fungi: Potential substrate for bioremediation",

abstract = "The composition of Scots pine bark, its degradation, and the production of hydrolytic and ligninolytic enzymes were evaluated during 90 days of incubation with Phanerochaete velutina and Stropharia rugosoannulata. The aim was to evaluate if pine bark can be a suitable fungal substrate for bioremediation applications. The original pine bark contained 45\% lignin, 25\% cellulose, and 15\% hemicellulose. Resin acids were the most predominant lipophilic extractives, followed by sitosterol and unsaturated fatty acids, such as linoleic and oleic acids. Both fungi degraded all main components of bark, specially cellulose (79\% loss by P. velutina). During cultivation on pine bark, fungi also degraded sitosterol, produced malic acid, and oxidated unsaturated fatty acids. The most predominant enzymes produced by both fungi were cellulase and manganese peroxidase. The results indicate that Scots pine bark supports enzyme production and provides nutrients to fungi, thus pine bark may be suitable fungal substrate for bioremediation.",

keywords = "Extractives, Hydrolytic enzymes, Manganese peroxidase, Phanerochaete velutina, Stropharia rugosoannulata",

author = "Lara Valent{\'i}n and Beata Kluczek-Turpeinen and Stefan Willf{\"o}r and Jarl Hemming and Annele Hatakka and Kari Steffen and Marja Tuomela",

note = "Funding Information: Author Lara Valent{\'i}n thanks the Finnish Graduate School of Environmental Science and Technology (EnSTe) for supporting her PhD studies. This study was funded by Niska ja Nyyss{\"o}nen Oy and by the Finnish Funding Agency for Technology and Innovation (TEKES) through SYMBIO program. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.biortech.2009.11.052",

language = "English",

volume = "101",

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AU - Valentín, Lara

AU - Kluczek-Turpeinen, Beata

AU - Willför, Stefan

AU - Hemming, Jarl

AU - Hatakka, Annele

AU - Steffen, Kari

AU - Tuomela, Marja

N1 - Funding Information: Author Lara Valentín thanks the Finnish Graduate School of Environmental Science and Technology (EnSTe) for supporting her PhD studies. This study was funded by Niska ja Nyyssönen Oy and by the Finnish Funding Agency for Technology and Innovation (TEKES) through SYMBIO program. Copyright: Copyright 2017 Elsevier B.V., All rights reserved.

PY - 2010/4

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N2 - The composition of Scots pine bark, its degradation, and the production of hydrolytic and ligninolytic enzymes were evaluated during 90 days of incubation with Phanerochaete velutina and Stropharia rugosoannulata. The aim was to evaluate if pine bark can be a suitable fungal substrate for bioremediation applications. The original pine bark contained 45% lignin, 25% cellulose, and 15% hemicellulose. Resin acids were the most predominant lipophilic extractives, followed by sitosterol and unsaturated fatty acids, such as linoleic and oleic acids. Both fungi degraded all main components of bark, specially cellulose (79% loss by P. velutina). During cultivation on pine bark, fungi also degraded sitosterol, produced malic acid, and oxidated unsaturated fatty acids. The most predominant enzymes produced by both fungi were cellulase and manganese peroxidase. The results indicate that Scots pine bark supports enzyme production and provides nutrients to fungi, thus pine bark may be suitable fungal substrate for bioremediation.

AB - The composition of Scots pine bark, its degradation, and the production of hydrolytic and ligninolytic enzymes were evaluated during 90 days of incubation with Phanerochaete velutina and Stropharia rugosoannulata. The aim was to evaluate if pine bark can be a suitable fungal substrate for bioremediation applications. The original pine bark contained 45% lignin, 25% cellulose, and 15% hemicellulose. Resin acids were the most predominant lipophilic extractives, followed by sitosterol and unsaturated fatty acids, such as linoleic and oleic acids. Both fungi degraded all main components of bark, specially cellulose (79% loss by P. velutina). During cultivation on pine bark, fungi also degraded sitosterol, produced malic acid, and oxidated unsaturated fatty acids. The most predominant enzymes produced by both fungi were cellulase and manganese peroxidase. The results indicate that Scots pine bark supports enzyme production and provides nutrients to fungi, thus pine bark may be suitable fungal substrate for bioremediation.

KW - Extractives

KW - Hydrolytic enzymes

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KW - Phanerochaete velutina

KW - Stropharia rugosoannulata

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Scots pine (Pinus sylvestris) bark composition and degradation by fungi: Potential substrate for bioremediation

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