Round Robin test on bio-imaging transfer standard for 3D optical profilers

A. Nolvi; T. Viitala; A. García Pérez; N. Sandler; E. Hæggström; C. Bermudez; R. Artigas; I. Kassamakov

doi:10.1117/12.2250261

Round Robin test on bio-imaging transfer standard for 3D optical profilers

A. Nolvi
, T. Viitala
, A. García Pérez
, N. Sandler
, E. Hæggström
, C. Bermudez
, R. Artigas
, I. Kassamakov

Research output: Chapter in Book/Conference proceeding › Published conference proceeding › Scientific › peer-review

1 Citation (Scopus)

Abstract

A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.

Original language	English
Title of host publication	Photonic Instrumentation Engineering IV
Editors	Yakov G. Soskind, Craig Olson
Publisher	SPIE, The International Society for Optical Engineering
ISBN (Electronic)	9781510606616
ISBN (Print)	9781510606616
DOIs	https://doi.org/10.1117/12.2250261
Publication status	Published - 2017
MoE publication type	A4 Article in a conference publication
Event	Photonic Instrumentation Engineering IV - San Francisco, California, United States Duration: 28 Jan 2017 → 2 Feb 2017

Publication series

Name	Proceedings of SPIE - The International Society for Optical Engineering
Volume	10110
ISSN (Print)	0277-786X
ISSN (Electronic)	1996-756X

Conference

Conference	Photonic Instrumentation Engineering IV
Country/Territory	United States
City	San Francisco, California
Period	28/01/17 → 02/02/17

Keywords

Bio transfer standard
Coherence Scanning Interferometry
Confocal
Optical Profiler
Phase Shifting Interferometry

Access to Document

10.1117/12.2250261

https://www.spiedigitallibrary.org/conference-proceedings-of-spie/10110/101100M/Round-Robin-test-on-bio-imaging-transfer-standard-for-3D/10.1117/12.2250261.short

Cite this

Nolvi, A., Viitala, T., García Pérez, A., Sandler, N., Hæggström, E., Bermudez, C., Artigas, R., & Kassamakov, I. (2017). Round Robin test on bio-imaging transfer standard for 3D optical profilers. In Y. G. Soskind, & C. Olson (Eds.), Photonic Instrumentation Engineering IV Article 101100M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10110). SPIE, The International Society for Optical Engineering. https://doi.org/10.1117/12.2250261

@inproceedings{3ba49b648ce0417c8f80350207d23757,

title = "Round Robin test on bio-imaging transfer standard for 3D optical profilers",

abstract = "A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.",

keywords = "Bio transfer standard, Coherence Scanning Interferometry, Confocal, Optical Profiler, Phase Shifting Interferometry",

author = "A. Nolvi and T. Viitala and \{Garc{\'i}a P{\'e}rez\}, A. and N. Sandler and E. H{\ae}ggstr{\"o}m and C. Bermudez and R. Artigas and I. Kassamakov",

note = "Publisher Copyright: {\textcopyright} COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.; Photonic Instrumentation Engineering IV ; Conference date: 28-01-2017 Through 02-02-2017",

year = "2017",

doi = "10.1117/12.2250261",

language = "English",

isbn = "9781510606616",

series = "Proceedings of SPIE - The International Society for Optical Engineering",

publisher = "SPIE, The International Society for Optical Engineering",

editor = "Soskind, \{Yakov G.\} and Craig Olson",

booktitle = "Photonic Instrumentation Engineering IV",

}

Nolvi, A, Viitala, T, García Pérez, A, Sandler, N, Hæggström, E, Bermudez, C, Artigas, R & Kassamakov, I 2017, Round Robin test on bio-imaging transfer standard for 3D optical profilers. in YG Soskind & C Olson (eds), Photonic Instrumentation Engineering IV., 101100M, Proceedings of SPIE - The International Society for Optical Engineering, vol. 10110, SPIE, The International Society for Optical Engineering, Photonic Instrumentation Engineering IV, San Francisco, California, United States, 28/01/17. https://doi.org/10.1117/12.2250261

Round Robin test on bio-imaging transfer standard for 3D optical profilers. / Nolvi, A.; Viitala, T.; García Pérez, A. et al.
Photonic Instrumentation Engineering IV. ed. / Yakov G. Soskind; Craig Olson. SPIE, The International Society for Optical Engineering, 2017. 101100M (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 10110).

Research output: Chapter in Book/Conference proceeding › Published conference proceeding › Scientific › peer-review

TY - GEN

T1 - Round Robin test on bio-imaging transfer standard for 3D optical profilers

AU - Nolvi, A.

AU - Viitala, T.

AU - García Pérez, A.

AU - Sandler, N.

AU - Hæggström, E.

AU - Bermudez, C.

AU - Artigas, R.

AU - Kassamakov, I.

N1 - Publisher Copyright: © COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.

PY - 2017

Y1 - 2017

N2 - A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.

AB - A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.

KW - Bio transfer standard

KW - Coherence Scanning Interferometry

KW - Confocal

KW - Optical Profiler

KW - Phase Shifting Interferometry

UR - https://www.scopus.com/pages/publications/85018882646

U2 - 10.1117/12.2250261

DO - 10.1117/12.2250261

M3 - Published conference proceeding

SN - 9781510606616

T3 - Proceedings of SPIE - The International Society for Optical Engineering

BT - Photonic Instrumentation Engineering IV

A2 - Soskind, Yakov G.

A2 - Olson, Craig

PB - SPIE, The International Society for Optical Engineering

T2 - Photonic Instrumentation Engineering IV

Y2 - 28 January 2017 through 2 February 2017

ER -

Nolvi A, Viitala T, García Pérez A, Sandler N, Hæggström E, Bermudez C et al. Round Robin test on bio-imaging transfer standard for 3D optical profilers. In Soskind YG, Olson C, editors, Photonic Instrumentation Engineering IV. SPIE, The International Society for Optical Engineering. 2017. 101100M. (Proceedings of SPIE - The International Society for Optical Engineering). doi: 10.1117/12.2250261

Round Robin test on bio-imaging transfer standard for 3D optical profilers

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