Round Robin test on bio-imaging transfer standard for 3D optical profilers

A Nolvi, T Viitala, AG Pérez, Niklas Sandler, E Hæggström, C Bermudez, R Artigas, I Kassamakov

Research output: Chapter in Book/Conference proceedingConference contributionScientificpeer-review

1 Citation (Scopus)

Abstract

A stair case height Bio-Transfer-Standard (BTS), developed and produced at the University of Helsinki (UH), was measured in two laboratories. The Round Robin test aims to determine whether BTS works with different optical profilers in different laboratories. First the artefact was measured at UH using a custom-built Scanning White Light Interferometer. Then BTS was measured at Sensofar-Tech, S.L. using an S-neox-type interferometer working either in Phase Shifting Interferometry mode or in Imaging Confocal Microscopy mode. To remove the influence of system calibration, a method featuring sample shifting and measurement subtraction was used. The BTS features eight lipid bilayer steps that each are 4.6 ± 0.1 nm tall on average. All 30 measurements done by four different operators at the two laboratories agree to within 0.1 nm which agrees with theoretical estimates and with measurements done using a surface plasmon resonance technique. The Round Robin results show the applicability of the newly developed bio-imaging transfer standard for calibrating 3D optical profilers.

Original languageUndefined/Unknown
Title of host publicationProc. SPIE 10110, Photonic Instrumentation Engineering IV
EditorsYakov G. Soskind, Craig Olson
PublisherSPIE
Pages
ISBN (Print)9781510606616
DOIs
Publication statusPublished - 2017
MoE publication typeA4 Article in a conference publication
EventPhotonic Instrumentation Engineering - Photonic Instrumentation Engineering IV
Duration: 1 Jan 2017 → …

Conference

ConferencePhotonic Instrumentation Engineering
Period01/01/17 → …

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