Real-Time Label-Free Monitoring of Nanoparticle Cell Uptake

Teemu Suutari, Tiina Silen, Didem SŞen Karaman, Heikki Saari, Diti Desai, Erja Kerkelä, Saara Laitinen, Martina Hanzlikova, Jessica M. Rosenholm, Marjo Yliperttula, Tapani Viitala*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

16 Citations (Scopus)

Abstract

The surface plasmon resonance technique in combination with whole cell sensing is used for the first time for real-time label-free monitoring of nanoparticle cell uptake. The uptake kinetics of several types of nanoparticles relevant to drug delivery applications into HeLa cells is determined. The cell uptake of the nanoparticles is confirmed by confocal microscopy. The cell uptake of silica nanoparticles and polyethylenimine–plasmid DNA polyplexes is studied as a function of temperature, and the uptake energies are determined by Arrhenius plots. The phase transition temperature of the HeLa cell membrane is detected when monitoring cell uptake of silica nanoparticles at different temperatures. The HeLa cell uptake of the mesoporous silica nanoparticles is energy-independent at temperatures slightly higher than the phase transition temperature of the HeLa cell membrane, while the uptake of polyethylenimine–DNA polyplexes is energy-dependent and linear as a function of temperature with an activation energy of Ea = 62 ± 7 kJ mol−1 = 15 ± 2 kcal mol−1. The HeLa cell uptake of red blood cell derived extracellular vesicles is also studied as a function of the extracellular vesicle concentration. The results show a concentration dependent behavior reaching a saturation level of the extracellular vesicle uptake by HeLa cells.

Original languageEnglish
Pages (from-to)6289-6300
Number of pages12
JournalSmall
Volume12
Issue number45
DOIs
Publication statusPublished - 7 Dec 2016
MoE publication typeA1 Journal article-refereed

Keywords

  • cell uptake
  • HeLa cells
  • kinetics
  • nanoparticles
  • resonance
  • surface plasmon

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