Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies

Ezgi Özliseli; Didem Ṣen Karaman; Soumyananda Chakraborti; Anna Slita; Marjaana Parikainen; Cecilia M. Sahlgren; Jessica M. Rosenholm

doi:10.1016/j.colsurfa.2020.124945

Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies

Ezgi Özliseli, Didem Ṣen Karaman^*, Soumyananda Chakraborti, Anna Slita, Marjaana Parikainen, Cecilia M. Sahlgren, Jessica M. Rosenholm

^*Corresponding author for this work

Research output: Contribution to journal › Article › Scientific › peer-review

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Abstract

The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.

Original language	English
Article number	124945
Journal	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volume	600
DOIs	https://doi.org/10.1016/j.colsurfa.2020.124945
Publication status	Published - 5 Sept 2020
MoE publication type	A1 Journal article-refereed

Funding

Sigrid Jusélius Foundation (Finland) , Swedish Cultural Foundation (Finland) , and Doctoral Network for Molecular Biosciences of Åbo Akademi University are gratefully acknowledged providing financial support to E.Ö. D.S.K. thankfully acknowledge the researching funding (#2018-GAP-MÜMF-0008) from Scientific Research Projects Coordination Unit of Izmir Katip Çelebi University (IKCU), additional to the support provided by Biomedical Engineering Central Research Laboratories of Izmir Kâtip Çelebi University . -. C.S. and M.P. are supported by The Academy of Finland (# 307133). K. Albin Johansson Foundation is acknowledged for financial support to M.P. The Academy of Finland (# 309374 ) is also greatly acknowledged for financial support to J . M.R.

Keywords

Cellular uptake
Copolymer coatings
Human serum albumin binding
Mesoporous silica nanoparticles
Stem cell therapy

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10.1016/j.colsurfa.2020.124945

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https://urn.fi/URN:NBN:fi-fe202201147737

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Özliseli, E., Ṣen Karaman, D., Chakraborti, S., Slita, A., Parikainen, M., Sahlgren, C. M., & Rosenholm, J. M. (2020). Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 600, Article 124945. https://doi.org/10.1016/j.colsurfa.2020.124945

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title = "Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies",

abstract = "The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.",

keywords = "Cellular uptake, Copolymer coatings, Human serum albumin binding, Mesoporous silica nanoparticles, Stem cell therapy",

author = "Ezgi {\"O}zliseli and \{Ṣen Karaman\}, Didem and Soumyananda Chakraborti and Anna Slita and Marjaana Parikainen and Sahlgren, \{Cecilia M.\} and Rosenholm, \{Jessica M.\}",

note = "Funding Information: Sigrid Jus{\'e}lius Foundation (Finland) , Swedish Cultural Foundation (Finland) , and Doctoral Network for Molecular Biosciences of {\AA}bo Akademi University are gratefully acknowledged providing financial support to E.{\"O}. D.S.K. thankfully acknowledge the researching funding (\#2018-GAP-M{\"U}MF-0008) from Scientific Research Projects Coordination Unit of Izmir Katip {\c C}elebi University (IKCU), additional to the support provided by Biomedical Engineering Central Research Laboratories of Izmir K{\^a}tip {\c C}elebi University . -. C.S. and M.P. are supported by The Academy of Finland (\# 307133). K. Albin Johansson Foundation is acknowledged for financial support to M.P. The Academy of Finland (\# 309374 ) is also greatly acknowledged for financial support to J . M.R. Publisher Copyright: {\textcopyright} 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.",

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doi = "10.1016/j.colsurfa.2020.124945",

language = "English",

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Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies. / Özliseli, Ezgi; Ṣen Karaman, Didem; Chakraborti, Soumyananda et al.
In: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 600, 124945, 05.09.2020.

Research output: Contribution to journal › Article › Scientific › peer-review

TY - JOUR

T1 - Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies

AU - Özliseli, Ezgi

AU - Ṣen Karaman, Didem

AU - Chakraborti, Soumyananda

AU - Slita, Anna

AU - Parikainen, Marjaana

AU - Sahlgren, Cecilia M.

AU - Rosenholm, Jessica M.

N1 - Funding Information: Sigrid Jusélius Foundation (Finland) , Swedish Cultural Foundation (Finland) , and Doctoral Network for Molecular Biosciences of Åbo Akademi University are gratefully acknowledged providing financial support to E.Ö. D.S.K. thankfully acknowledge the researching funding (#2018-GAP-MÜMF-0008) from Scientific Research Projects Coordination Unit of Izmir Katip Çelebi University (IKCU), additional to the support provided by Biomedical Engineering Central Research Laboratories of Izmir Kâtip Çelebi University . -. C.S. and M.P. are supported by The Academy of Finland (# 307133). K. Albin Johansson Foundation is acknowledged for financial support to M.P. The Academy of Finland (# 309374 ) is also greatly acknowledged for financial support to J . M.R. Publisher Copyright: © 2020 Elsevier B.V. Copyright: Copyright 2020 Elsevier B.V., All rights reserved.

PY - 2020/9/5

Y1 - 2020/9/5

N2 - The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.

AB - The supplementation of stem cell culture medium with fetal bovine serum (FBS) for maintenance and propagation of cell lines in vitro is associated with immunogenicity and disease transmission by prions, bacteria, and viruses. Therefore, establishing xenogenic-free cell culture media for the benefit of rational testing conditions for stem cells in terms of eliminating the disadvantages is essential. In parallel, a number of investigations have been carried out on nanoparticle (NP) aided stem cell-based therapies. To use NP-integrated stem cell therapy in clinical applications, NP behavior in xenogenic-free stem cell cultures needs to be understood in detail. Mesoporous silica nanoparticles (MSN) are profusely used in biomedical applications and have also shown great potential in stem cell therapies. One of the strategies to make them compatible with stem cell therapy is to alter their surface functionalization. In line with this notion, the main interest of the present investigation was to study the impact of human serum albumin (HSA) association with differently surface-modified MSN, and rationalize the MSN utilization in xenogenic-free stem cell culture by employing C2C12 myogenic progenitor cells as a model system. Our results revealed that HSA coating on differently surface-modified MSN is a promising strategy to improve the colloidal stability of MSN, stem cell viability, and imparts no adverse effects on the differentiation of stem cells.

KW - Cellular uptake

KW - Copolymer coatings

KW - Human serum albumin binding

KW - Mesoporous silica nanoparticles

KW - Stem cell therapy

UR - https://www.scopus.com/pages/publications/85084940304

U2 - 10.1016/j.colsurfa.2020.124945

DO - 10.1016/j.colsurfa.2020.124945

M3 - Article

AN - SCOPUS:85084940304

SN - 0927-7757

VL - 600

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

M1 - 124945

ER -

Rational evaluation of human serum albumin coated mesoporous silica nanoparticles for xenogenic-free stem cell therapies

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Keywords

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