Rapid separation of microcystins and nodularin using a monolithic silica C18 column

Lisa Spoof; Jussi Meriluoto

doi:10.1016/S0021-9673(01)01605-3

Rapid separation of microcystins and nodularin using a monolithic silica C₁₈ column

Lisa Spoof, Jussi Meriluoto^*

^*Corresponding author for this work

Laboratoryservices

Research output: Contribution to journal › Article › Scientific › peer-review

61 Citations (Scopus)

Abstract

A monolithic C₁₈-bonded silica rod column (Merck Chromolith) was compared to particle-based C₁₈ and amide C₁₆ sorbents in the HPLC separation of eight microcystins and nodularin-R. Two gradient mobile phases of aqueous trifluoroacetic acid modified with acetonitrile or methanol, different flow-rates and different gradient lengths were tested. The performance of the Chromolith column measured as the resolution of some microcystin pairs, the selectivity, efficiency (peak width) and peak asymmetry equalled, or exceeded, the performance of traditional particle-based columns. The Chromolith column allowed a shortening of the total analysis time to 4.3 min with a flow-rate 4 ml min^-1.

Original language	English
Pages (from-to)	237-245
Number of pages	9
Journal	Journal of Chromatography A
Volume	947
Issue number	2
DOIs	https://doi.org/10.1016/S0021-9673(01)01605-3
Publication status	Published - 22 Feb 2002
MoE publication type	A1 Journal article-refereed

Funding

The authors wish to thank the Magnus Ehrnrooth Foundation, the Oskar Öflund Foundation and the Academy of Finland, RC for Natural Sciences and Engineering (project 47664) for financial support.

Keywords

Microcystins
Monolithic columns
Nodularin
Peptides
Stationary phases, LC
Toxins

Access to Document

10.1016/S0021-9673(01)01605-3

Cite this

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title = "Rapid separation of microcystins and nodularin using a monolithic silica C18 column",

abstract = "A monolithic C18-bonded silica rod column (Merck Chromolith) was compared to particle-based C18 and amide C16 sorbents in the HPLC separation of eight microcystins and nodularin-R. Two gradient mobile phases of aqueous trifluoroacetic acid modified with acetonitrile or methanol, different flow-rates and different gradient lengths were tested. The performance of the Chromolith column measured as the resolution of some microcystin pairs, the selectivity, efficiency (peak width) and peak asymmetry equalled, or exceeded, the performance of traditional particle-based columns. The Chromolith column allowed a shortening of the total analysis time to 4.3 min with a flow-rate 4 ml min-1.",

keywords = "Microcystins, Monolithic columns, Nodularin, Peptides, Stationary phases, LC, Toxins",

author = "Lisa Spoof and Jussi Meriluoto",

note = "Funding Information: The authors wish to thank the Magnus Ehrnrooth Foundation, the Oskar {\"O}flund Foundation and the Academy of Finland, RC for Natural Sciences and Engineering (project 47664) for financial support. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.",

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AU - Spoof, Lisa

AU - Meriluoto, Jussi

N1 - Funding Information: The authors wish to thank the Magnus Ehrnrooth Foundation, the Oskar Öflund Foundation and the Academy of Finland, RC for Natural Sciences and Engineering (project 47664) for financial support. Copyright: Copyright 2008 Elsevier B.V., All rights reserved.

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N2 - A monolithic C18-bonded silica rod column (Merck Chromolith) was compared to particle-based C18 and amide C16 sorbents in the HPLC separation of eight microcystins and nodularin-R. Two gradient mobile phases of aqueous trifluoroacetic acid modified with acetonitrile or methanol, different flow-rates and different gradient lengths were tested. The performance of the Chromolith column measured as the resolution of some microcystin pairs, the selectivity, efficiency (peak width) and peak asymmetry equalled, or exceeded, the performance of traditional particle-based columns. The Chromolith column allowed a shortening of the total analysis time to 4.3 min with a flow-rate 4 ml min-1.

AB - A monolithic C18-bonded silica rod column (Merck Chromolith) was compared to particle-based C18 and amide C16 sorbents in the HPLC separation of eight microcystins and nodularin-R. Two gradient mobile phases of aqueous trifluoroacetic acid modified with acetonitrile or methanol, different flow-rates and different gradient lengths were tested. The performance of the Chromolith column measured as the resolution of some microcystin pairs, the selectivity, efficiency (peak width) and peak asymmetry equalled, or exceeded, the performance of traditional particle-based columns. The Chromolith column allowed a shortening of the total analysis time to 4.3 min with a flow-rate 4 ml min-1.

KW - Microcystins

KW - Monolithic columns

KW - Nodularin

KW - Peptides

KW - Stationary phases, LC

KW - Toxins

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