Rapid separation of microcystins and nodularin using a monolithic silica C18 column

Lisa Spoof, Jussi Meriluoto*

*Corresponding author for this work

Research output: Contribution to journalArticleScientificpeer-review

58 Citations (Scopus)

Abstract

A monolithic C18-bonded silica rod column (Merck Chromolith) was compared to particle-based C18 and amide C16 sorbents in the HPLC separation of eight microcystins and nodularin-R. Two gradient mobile phases of aqueous trifluoroacetic acid modified with acetonitrile or methanol, different flow-rates and different gradient lengths were tested. The performance of the Chromolith column measured as the resolution of some microcystin pairs, the selectivity, efficiency (peak width) and peak asymmetry equalled, or exceeded, the performance of traditional particle-based columns. The Chromolith column allowed a shortening of the total analysis time to 4.3 min with a flow-rate 4 ml min-1.

Original languageEnglish
Pages (from-to)237-245
Number of pages9
JournalJournal of Chromatography A
Volume947
Issue number2
DOIs
Publication statusPublished - 22 Feb 2002
MoE publication typeA1 Journal article-refereed

Keywords

  • Microcystins
  • Monolithic columns
  • Nodularin
  • Peptides
  • Stationary phases, LC
  • Toxins

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