TY - JOUR
T1 - Rapid LC-MS detection of cyanobacterial hepatotoxins microcystins and nodularins-Comparison of columns
AU - Neffling, Milla Riina
AU - Spoof, Lisa
AU - Meriluoto, Jussi
N1 - Funding Information:
The authors would like to gratefully acknowledge financial support from Academy of Finland (decision number 108947), Tekes (decision number 40366/06), Otto A. Malm's donationsfond, Medicinska Understödsföreningen Liv och Hälsa r.f , and the Graduate School in Informational and Structural Biology (M-R N) . Dr. Michael Quilliam, NRC-IMB, Halifax, Canada is thanked for his expert advice during the early stages of planning of this study.
Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2009/10/27
Y1 - 2009/10/27
N2 - Eight reversed-phase columns intended for rapid HPLC were assessed for the separation of thirteen microcystins and nodularins, cyclic peptidic hepatotoxins. The instrumentation consisted of an Agilent Technologies 1200 Rapid Resolution high performance liquid chromatography system coupled to a mass spectrometer, Bruker Daltonics Ultra Performance High Capacity Ion Trap MS (HCT Ultra) with electrospray ionisation (RRLC-ESI-IT-MS). The columns tested were 2-2.1 mm × 50 mm in diameter and length, and contained small particles (1.8-2.7 μm), or monolithic silica supports for fast performance. The shortest total run time achieved was 3 min 15 s including equilibration and injection. Critical microcystin pairs were still resolved. Several columns showed excellent performance.
AB - Eight reversed-phase columns intended for rapid HPLC were assessed for the separation of thirteen microcystins and nodularins, cyclic peptidic hepatotoxins. The instrumentation consisted of an Agilent Technologies 1200 Rapid Resolution high performance liquid chromatography system coupled to a mass spectrometer, Bruker Daltonics Ultra Performance High Capacity Ion Trap MS (HCT Ultra) with electrospray ionisation (RRLC-ESI-IT-MS). The columns tested were 2-2.1 mm × 50 mm in diameter and length, and contained small particles (1.8-2.7 μm), or monolithic silica supports for fast performance. The shortest total run time achieved was 3 min 15 s including equilibration and injection. Critical microcystin pairs were still resolved. Several columns showed excellent performance.
KW - Cyclic peptides
KW - Liquid chromatography - mass spectrometry
KW - Microcystins
KW - Nodularins
KW - Toxins
KW - Ultra-high performance liquid chromatography systems
UR - http://www.scopus.com/inward/record.url?scp=71549162040&partnerID=8YFLogxK
U2 - 10.1016/j.aca.2009.09.015
DO - 10.1016/j.aca.2009.09.015
M3 - Article
C2 - 19808120
AN - SCOPUS:71549162040
SN - 0003-2670
VL - 653
SP - 234
EP - 241
JO - Analytica Chimica Acta
JF - Analytica Chimica Acta
IS - 2
ER -