PV-1 is recognized by the PAL-E antibody and forms complexes with NRP-1

Johannes Keuschnigg, Denis Tvorogov, Kati Elima, Marko Salmi, Kari Alitalo, Tiina Salminen, Sirpa Jalkanen

Research output: Contribution to journalArticleScientificpeer-review

11 Citations (Scopus)

Abstract

Pathologische anatomie leiden endothelium (PAL-E) antibody has been used for more than 20 years as a prototype marker for vascular endothelium. The elusive target of this antibody has been claimed to be plasmalemma vesicle-associated protein-1 (PV-1) and neuropilin-1 (NRP-1). Using immunofluorescence, we show that PAL-E, anti-PV-1, anti-NRP-1, and anti-CD31 antibodies show largely identical staining patterns in the vasculature of different tissues. However, PV-1-transfected cells only bind PAL-E and anti-PV-1 antibodies, whereas NRP-1 transfectants stain with anti-NRP-1 antibodies in flow cytometry. Using lysates from tissues and transfected cells, we further confirm that the molecule recognized by PAL-E and anti-PV-1 antibodies is not NRP-1 but PV-1. Nevertheless, coimmunoprecipitation studies unambiguously demonstrate that NRP-1 can form complexes with PV-1. This connects, for the first time, 2 molecules involved in leukocyte trafficking and angiogenesis, thereby opening interesting possibilities for future research in this field.

Original languageEnglish
Pages (from-to)232-5
Number of pages4
JournalBlood
Volume120
Issue number1
DOIs
Publication statusPublished - 5 Jul 2012
MoE publication typeA1 Journal article-refereed

Keywords

  • Antibodies/immunology
  • Antibody Specificity/immunology
  • Carrier Proteins/immunology
  • Endothelium, Lymphatic/immunology
  • Endothelium, Vascular/immunology
  • Fluorescent Antibody Technique
  • Humans
  • Membrane Proteins/immunology
  • Neuropilin-1/immunology

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