PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth

Jianmin Yang; Maria Lindahl; Päivi Lindholm; Heidi Virtanen; Eleanor Coffey; Pia Runeberg-Roos; Mart Saarma

doi:10.1016/j.febslet.2004.06.007

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth

Jianmin Yang, Maria Lindahl, Päivi Lindholm, Heidi Virtanen, Eleanor Coffey, Pia Runeberg-Roos, Mart Saarma

Turku Bioscience Centre

Research output: Contribution to journal › Article › Scientific › peer-review

16 Citations (Scopus)

Abstract

Previously, it was shown that the recruitment of RET into lipid rafts by glial cell line-derived neurotrophic factor (GDNF)/GFRalpha1 is crucial for efficient signal transduction. Here, we show that the mouse GFRalpha4 is a functional, N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored protein, which mediates persephin (PSPN)-induced phosphorylation of RET, but has an almost undetectable capacity to recruit RET into the 0.1% Triton X-100 insoluble membrane fraction. In spite of this, PSPN/mGFRalpha4 promotes neurite outgrowth in PC6-3 cells and survival of cerebellar granule neurons. As we show that also human PSPN/GFRalpha4 is unable to recruit RET into lipid rafts, we propose that the mammalian GFRalpha4 in this respect differs from GFRalpha1.

Original language	English
Pages (from-to)	267-71
Number of pages	5
Journal	FEBS Letters
Volume	569
Issue number	1-3
DOIs	https://doi.org/10.1016/j.febslet.2004.06.007
Publication status	Published - 2 Jul 2004
MoE publication type	A1 Journal article-refereed

Keywords

Animals
Cell Line
Cell Membrane/physiology
Cloning, Molecular
Glial Cell Line-Derived Neurotrophic Factor
Glial Cell Line-Derived Neurotrophic Factor Receptors
Humans
Membrane Glycoproteins/genetics
Membrane Microdomains/metabolism
Mice
Nerve Growth Factors/metabolism
Nerve Tissue Proteins/metabolism
Neurites/physiology
Neurons/cytology
Proto-Oncogene Proteins/genetics
Proto-Oncogene Proteins c-ret
Rats
Receptor Protein-Tyrosine Kinases/genetics
Receptors, Nerve Growth Factor/genetics
Recombinant Proteins/metabolism
Transfection

Access to Document

10.1016/j.febslet.2004.06.007

Cite this

@article{41cac033eee340b69283eb086e92cb0f,

title = "PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth",

abstract = "Previously, it was shown that the recruitment of RET into lipid rafts by glial cell line-derived neurotrophic factor (GDNF)/GFRalpha1 is crucial for efficient signal transduction. Here, we show that the mouse GFRalpha4 is a functional, N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored protein, which mediates persephin (PSPN)-induced phosphorylation of RET, but has an almost undetectable capacity to recruit RET into the 0.1% Triton X-100 insoluble membrane fraction. In spite of this, PSPN/mGFRalpha4 promotes neurite outgrowth in PC6-3 cells and survival of cerebellar granule neurons. As we show that also human PSPN/GFRalpha4 is unable to recruit RET into lipid rafts, we propose that the mammalian GFRalpha4 in this respect differs from GFRalpha1.",

keywords = "Animals, Cell Line, Cell Membrane/physiology, Cloning, Molecular, Glial Cell Line-Derived Neurotrophic Factor, Glial Cell Line-Derived Neurotrophic Factor Receptors, Humans, Membrane Glycoproteins/genetics, Membrane Microdomains/metabolism, Mice, Nerve Growth Factors/metabolism, Nerve Tissue Proteins/metabolism, Neurites/physiology, Neurons/cytology, Proto-Oncogene Proteins/genetics, Proto-Oncogene Proteins c-ret, Rats, Receptor Protein-Tyrosine Kinases/genetics, Receptors, Nerve Growth Factor/genetics, Recombinant Proteins/metabolism, Transfection",

author = "Jianmin Yang and Maria Lindahl and P{\"a}ivi Lindholm and Heidi Virtanen and Eleanor Coffey and Pia Runeberg-Roos and Mart Saarma",

year = "2004",

month = jul,

day = "2",

doi = "10.1016/j.febslet.2004.06.007",

language = "English",

volume = "569",

pages = "267--71",

journal = "FEBS Letters",

issn = "0014-5793",

publisher = "Elsevier",

number = "1-3",

}

TY - JOUR

T1 - PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth

AU - Yang, Jianmin

AU - Lindahl, Maria

AU - Lindholm, Päivi

AU - Virtanen, Heidi

AU - Coffey, Eleanor

AU - Runeberg-Roos, Pia

AU - Saarma, Mart

PY - 2004/7/2

Y1 - 2004/7/2

N2 - Previously, it was shown that the recruitment of RET into lipid rafts by glial cell line-derived neurotrophic factor (GDNF)/GFRalpha1 is crucial for efficient signal transduction. Here, we show that the mouse GFRalpha4 is a functional, N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored protein, which mediates persephin (PSPN)-induced phosphorylation of RET, but has an almost undetectable capacity to recruit RET into the 0.1% Triton X-100 insoluble membrane fraction. In spite of this, PSPN/mGFRalpha4 promotes neurite outgrowth in PC6-3 cells and survival of cerebellar granule neurons. As we show that also human PSPN/GFRalpha4 is unable to recruit RET into lipid rafts, we propose that the mammalian GFRalpha4 in this respect differs from GFRalpha1.

AB - Previously, it was shown that the recruitment of RET into lipid rafts by glial cell line-derived neurotrophic factor (GDNF)/GFRalpha1 is crucial for efficient signal transduction. Here, we show that the mouse GFRalpha4 is a functional, N-glycosylated, glycosylphosphatidylinositol (GPI)-anchored protein, which mediates persephin (PSPN)-induced phosphorylation of RET, but has an almost undetectable capacity to recruit RET into the 0.1% Triton X-100 insoluble membrane fraction. In spite of this, PSPN/mGFRalpha4 promotes neurite outgrowth in PC6-3 cells and survival of cerebellar granule neurons. As we show that also human PSPN/GFRalpha4 is unable to recruit RET into lipid rafts, we propose that the mammalian GFRalpha4 in this respect differs from GFRalpha1.

KW - Animals

KW - Cell Line

KW - Cell Membrane/physiology

KW - Cloning, Molecular

KW - Glial Cell Line-Derived Neurotrophic Factor

KW - Glial Cell Line-Derived Neurotrophic Factor Receptors

KW - Humans

KW - Membrane Glycoproteins/genetics

KW - Membrane Microdomains/metabolism

KW - Mice

KW - Nerve Growth Factors/metabolism

KW - Nerve Tissue Proteins/metabolism

KW - Neurites/physiology

KW - Neurons/cytology

KW - Proto-Oncogene Proteins/genetics

KW - Proto-Oncogene Proteins c-ret

KW - Rats

KW - Receptor Protein-Tyrosine Kinases/genetics

KW - Receptors, Nerve Growth Factor/genetics

KW - Recombinant Proteins/metabolism

KW - Transfection

U2 - 10.1016/j.febslet.2004.06.007

DO - 10.1016/j.febslet.2004.06.007

M3 - Article

C2 - 15225646

SN - 0014-5793

VL - 569

SP - 267

EP - 271

JO - FEBS Letters

JF - FEBS Letters

IS - 1-3

ER -

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth

Abstract

Keywords

Access to Document

Fingerprint

Cite this