TY - JOUR
T1 - Proteome variance differences within populations of European whitefish (Coregonus lavaretus) originating from contrasting salinity environments
AU - Papakostas, S
AU - Vasemägi, A
AU - Himberg, Mikael
AU - Primmer, CR
PY - 2014
Y1 - 2014
N2 - Variation in gene expression is an important component of the phenotypic differences observed in nature. Gene expression variance across biological groups and environmental conditions has been studied extensively and has revealed specific genes and molecular mechanisms of interest. However, little is known regarding the importance of within-population gene expression variation to environmental adaptation. To address this issue, we quantified the proteomes of individuals of European whitefish (Coregonus lavaretus) from populations that have previously been shown to have adapted during early development to freshwater and brackishwater salinity environments. Using MS-based label-free proteomics, we studied 955 proteins in eight hatch-stage fish embryos from each population that had been reared in either freshwater or brackishwater salinity conditions. By comparing the levels of within-population protein expression variance over individuals and per protein between populations, we found that fish embryos from the population less affected by salinity level had also markedly higher levels of expression variance. Gene Ontologies and molecular pathways associated with osmoregulation showed the most significant difference of within-population proteome variance between populations. Several new candidate genes for salinity adaptation were identified, emphasising the added value of combining assessments of within-population gene expression variation with standard gene expression analysis practices for better understanding the mechanisms of environmental adaptation.
AB - Variation in gene expression is an important component of the phenotypic differences observed in nature. Gene expression variance across biological groups and environmental conditions has been studied extensively and has revealed specific genes and molecular mechanisms of interest. However, little is known regarding the importance of within-population gene expression variation to environmental adaptation. To address this issue, we quantified the proteomes of individuals of European whitefish (Coregonus lavaretus) from populations that have previously been shown to have adapted during early development to freshwater and brackishwater salinity environments. Using MS-based label-free proteomics, we studied 955 proteins in eight hatch-stage fish embryos from each population that had been reared in either freshwater or brackishwater salinity conditions. By comparing the levels of within-population protein expression variance over individuals and per protein between populations, we found that fish embryos from the population less affected by salinity level had also markedly higher levels of expression variance. Gene Ontologies and molecular pathways associated with osmoregulation showed the most significant difference of within-population proteome variance between populations. Several new candidate genes for salinity adaptation were identified, emphasising the added value of combining assessments of within-population gene expression variation with standard gene expression analysis practices for better understanding the mechanisms of environmental adaptation.
KW - Baltic Sea
KW - Early-life development
KW - G-protein coupled receptors
KW - Label-free proteomics
KW - Salinity stress
KW - Baltic Sea
KW - Early-life development
KW - G-protein coupled receptors
KW - Label-free proteomics
KW - Salinity stress
KW - Baltic Sea
KW - Early-life development
KW - G-protein coupled receptors
KW - Label-free proteomics
KW - Salinity stress
U2 - 10.1016/j.jprot.2013.12.019
DO - 10.1016/j.jprot.2013.12.019
M3 - Artikel
SN - 1874-3919
VL - 105
SP - 144
EP - 150
JO - Journal of Proteomics
JF - Journal of Proteomics
ER -