Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

ME Sandberg; D Schellmann; G Brunhofer; T Erker; I Busygin; Reko Leino; PM Vuorela; A Fallarero

doi:10.1016/j.mimet.2009.04.014

Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

ME Sandberg, D Schellmann, G Brunhofer, T Erker, I Busygin, Reko Leino, PM Vuorela, A Fallarero

Research output: Contribution to journal › Article › Scientific › peer-review

80 Citations (Scopus)

Abstract

Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

Original language	Undefined/Unknown
Pages (from-to)	104–106
Number of pages	3
Journal	Journal of Microbiological Methods
Volume	78
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2009.04.014
Publication status	Published - 2009
MoE publication type	A1 Journal article-refereed

Keywords

Biofilms
Resazurin
Screening
Staphylococcus aureus

Access to Document

10.1016/j.mimet.2009.04.014

Cite this

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title = "Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay",

abstract = "Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.",

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T1 - Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

AU - Sandberg, ME

AU - Schellmann, D

AU - Brunhofer, G

AU - Erker, T

AU - Busygin, I

AU - Leino, Reko

AU - Vuorela, PM

AU - Fallarero, A

PY - 2009

Y1 - 2009

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AB - Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

KW - Biofilms

KW - Resazurin

KW - Screening

KW - Staphylococcus aureus

U2 - 10.1016/j.mimet.2009.04.014

DO - 10.1016/j.mimet.2009.04.014

M3 - Artikel

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SP - 104

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JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

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ER -