Pros and cons of using resazurin staining for quantification of viable Staphylococcus aureus biofilms in a screening assay

ME Sandberg, D Schellmann, G Brunhofer, T Erker, I Busygin, Reko Leino, PM Vuorela, A Fallarero

    Research output: Contribution to journalArticleScientificpeer-review

    51 Citations (Scopus)

    Abstract

    Staining of Staphylococcus aureus biofilms with 20 mu M resazurin during 20 min was shown to provide a good screening assay in 96-well micro titer plates. However, data quality was found to be dependent on the staining duration and biofilm concentration. Also, the inadequacy of using resazurin calibration curves with planktonic cells to estimate S. aureus biofilm concentrations was demonstrated. (C) 2009 Elsevier B.V. All rights reserved.
    Original languageUndefined/Unknown
    Pages (from-to)104–106
    Number of pages3
    JournalJournal of Microbiological Methods
    Volume78
    Issue number1
    DOIs
    Publication statusPublished - 2009
    MoE publication typeA1 Journal article-refereed

    Keywords

    • Biofilms
    • Resazurin
    • Screening
    • Staphylococcus aureus

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