Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine

null Mikhailov; Ann-Sofi Härmälä-Brasken; Jussi Meriluoto; null Sorokina; null Dietrich; John Eriksson

Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine

Mikhailov, Ann-Sofi Härmälä-Brasken, Jussi Meriluoto, Sorokina, Dietrich, John Eriksson

Research output: Contribution to journal › Article › Scientific › peer-review

35 Citations (Scopus)

Abstract

Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.

Original language	Undefined/Unknown
Pages (from-to)	477–483
Journal	Toxicon
Volume	39
Issue number	4
Publication status	Published - 2001
MoE publication type	A1 Journal article-refereed

Access to Document

http://10.1016/S0041-0101(00)00148-3

Cite this

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title = "Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine",

abstract = "Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.",

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TY - JOUR

T1 - Production and specificity of mono and polyclonal antibodies against microcystins conjugated through N-methyldehydroalanine

AU - Mikhailov, null

AU - Härmälä-Brasken, Ann-Sofi

AU - Meriluoto, Jussi

AU - Sorokina, null

AU - Dietrich, null

AU - Eriksson, John

PY - 2001

Y1 - 2001

N2 - Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.

AB - Microcystins (MCs) are a group of closely related toxic cyclic heptapeptides produced by common cyanobacteria (blue-green algae). Their toxicity is associated with specific inhibition of intracellular protein phosphatases type-1 and type-2A (PP1 and PP2A, respectively). We have developed a battery of antibodies to microcystins using chemical modification (aminoethylation) of one of its core amino acids, N-methyl-dehydroalanine. The developed antibodies displayed different reactivities to closely related MCs. Selected monoclonal antibodies were used for quantitative competitive ELISA assays. The analytical sensitivity of these assays was up to 1 ng/ml. Comparison of the developed ELISA tests with HPLC-based measurements of MCs in laboratory and field samples showed a good correspondence between the results yielded by these two methods. The antibodies developed by this technique provide the means for developing extremely sensitive and specific analytical assays for direct measurement of toxins in cyanobacterial or water samples.

M3 - Artikel

SN - 0041-0101

VL - 39

SP - 477

EP - 483

JO - Toxicon

JF - Toxicon

IS - 4

ER -