TY - JOUR
T1 - Point-Of-Care Ultra-Portable Single-Molecule Bioassays for One-Health
AU - Macchia, Eleonora
AU - Torricelli, Fabrizio
AU - Caputo, Mariapia
AU - Sarcina, Lucia
AU - Scandurra, Cecilia
AU - Bollella, Paolo
AU - Catacchio, Michele
AU - Piscitelli, Matteo
AU - Di Franco, Cinzia
AU - Scamarcio, Gaetano
AU - Torsi, Luisa
N1 - Publisher Copyright:
© 2023 The Authors. Advanced Materials published by Wiley-VCH GmbH.
PY - 2024/3/28
Y1 - 2024/3/28
N2 - Screening asymptomatic organisms (humans, animals, plants) with a high-diagnostic accuracy using point-of-care-testing (POCT) technologies, though still visionary holds great potential. Convenient surveillance requires easy-to-use, cost-effective, ultra-portable but highly reliable, in-vitro-diagnostic devices that are ready for use wherever they are needed. Currently, there are not yet such devices available on the market, but there are a couple more promising technologies developed at readiness-level 5: the Clustered-Regularly-Interspaced-Short-Palindromic-Repeats (CRISPR) lateral-flow-strip tests and the Single-Molecule-with-a-large-Transistor (SiMoT) bioelectronic palmar devices. They both hold key features delineated by the World-Health-Organization for POCT systems and an occurrence of false-positive and false-negative errors <1–5% resulting in diagnostic-selectivity and sensitivity >95–99%, while limit-of-detections are of few markers. CRISPR-strip is a molecular assay that, can detect down to few copies of DNA/RNA markers in blood while SiMoT immunometric and molecular test can detect down to a single oligonucleotide, protein marker, or pathogens in 0.1mL of blood, saliva, and olive-sap. These technologies can prospectively enable the systematic and reliable surveillance of asymptomatic ones prior to worsening/proliferation of illnesses allowing for timely diagnosis and swift prognosis. This could establish a proactive healthcare ecosystem that results in effective treatments for all living organisms generating diffuse and well-being at efficient costs.
AB - Screening asymptomatic organisms (humans, animals, plants) with a high-diagnostic accuracy using point-of-care-testing (POCT) technologies, though still visionary holds great potential. Convenient surveillance requires easy-to-use, cost-effective, ultra-portable but highly reliable, in-vitro-diagnostic devices that are ready for use wherever they are needed. Currently, there are not yet such devices available on the market, but there are a couple more promising technologies developed at readiness-level 5: the Clustered-Regularly-Interspaced-Short-Palindromic-Repeats (CRISPR) lateral-flow-strip tests and the Single-Molecule-with-a-large-Transistor (SiMoT) bioelectronic palmar devices. They both hold key features delineated by the World-Health-Organization for POCT systems and an occurrence of false-positive and false-negative errors <1–5% resulting in diagnostic-selectivity and sensitivity >95–99%, while limit-of-detections are of few markers. CRISPR-strip is a molecular assay that, can detect down to few copies of DNA/RNA markers in blood while SiMoT immunometric and molecular test can detect down to a single oligonucleotide, protein marker, or pathogens in 0.1mL of blood, saliva, and olive-sap. These technologies can prospectively enable the systematic and reliable surveillance of asymptomatic ones prior to worsening/proliferation of illnesses allowing for timely diagnosis and swift prognosis. This could establish a proactive healthcare ecosystem that results in effective treatments for all living organisms generating diffuse and well-being at efficient costs.
KW - clustered regularly interspaced short palindromic repeats/Cas (CRISPR/Cas)
KW - lateral flow strip test
KW - one health
KW - point of care testing (POCT)
KW - preventing healthcare
KW - Single-molecule with a large transistor(SiMoT)
UR - http://www.scopus.com/inward/record.url?scp=85180656497&partnerID=8YFLogxK
U2 - 10.1002/adma.202309705
DO - 10.1002/adma.202309705
M3 - Review Article or Literature Review
AN - SCOPUS:85180656497
SN - 0935-9648
VL - 36
JO - Advanced Materials
JF - Advanced Materials
IS - 13
M1 - 2309705
ER -