Point-Of-Care Ultra-Portable Single-Molecule Bioassays for One-Health

Eleonora Macchia, Fabrizio Torricelli, Mariapia Caputo, Lucia Sarcina, Cecilia Scandurra, Paolo Bollella, Michele Catacchio, Matteo Piscitelli, Cinzia Di Franco, Gaetano Scamarcio, Luisa Torsi*

*Corresponding author for this work

Research output: Contribution to journalReview Article or Literature Reviewpeer-review

16 Citations (Scopus)

Abstract

Screening asymptomatic organisms (humans, animals, plants) with a high-diagnostic accuracy using point-of-care-testing (POCT) technologies, though still visionary holds great potential. Convenient surveillance requires easy-to-use, cost-effective, ultra-portable but highly reliable, in-vitro-diagnostic devices that are ready for use wherever they are needed. Currently, there are not yet such devices available on the market, but there are a couple more promising technologies developed at readiness-level 5: the Clustered-Regularly-Interspaced-Short-Palindromic-Repeats (CRISPR) lateral-flow-strip tests and the Single-Molecule-with-a-large-Transistor (SiMoT) bioelectronic palmar devices. They both hold key features delineated by the World-Health-Organization for POCT systems and an occurrence of false-positive and false-negative errors <1–5% resulting in diagnostic-selectivity and sensitivity >95–99%, while limit-of-detections are of few markers. CRISPR-strip is a molecular assay that, can detect down to few copies of DNA/RNA markers in blood while SiMoT immunometric and molecular test can detect down to a single oligonucleotide, protein marker, or pathogens in 0.1mL of blood, saliva, and olive-sap. These technologies can prospectively enable the systematic and reliable surveillance of asymptomatic ones prior to worsening/proliferation of illnesses allowing for timely diagnosis and swift prognosis. This could establish a proactive healthcare ecosystem that results in effective treatments for all living organisms generating diffuse and well-being at efficient costs.

Original languageEnglish
Article number2309705
JournalAdvanced Materials
Volume36
Issue number13
DOIs
Publication statusPublished - 28 Mar 2024
Externally publishedYes
MoE publication typeA2 Review article in a scientific journal

Keywords

  • clustered regularly interspaced short palindromic repeats/Cas (CRISPR/Cas)
  • lateral flow strip test
  • one health
  • point of care testing (POCT)
  • preventing healthcare
  • Single-molecule with a large transistor(SiMoT)

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